Difference between revisions of "Team:NYMU-Taipei/Notebook-Lab Book-pGFP C120 RFP"

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<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-C120_empty">C120_empty</a></h3></li>
 
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-C120_empty">C120_empty</a></h3></li>
  
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<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="ttp://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-minimal-PgpdA">minimal-PgpdA</a></h3></li>
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<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-minimal-PgpdA">minimal-PgpdA</a></h3></li>
  
 
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-RFP">RFP</a></h3></li>
 
<li style="list-style-type:disc;"><h3 style="margin-top:10px; margin-bottom:0px; line-height: 24px;"><a href="https://2016.igem.org/Team:NYMU-Taipei/Notebook-Lab_Book-RFP">RFP</a></h3></li>

Revision as of 18:59, 19 October 2016

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9/23

  • GFP_TtrpC ligation PCR

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    9/24

  • GFP_TtrpC ligation PCR

  • GFP_TtrpC ligation PCR (We confirmed junction primer was wrong)

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    9/30

  • Received the junction primer of GFP-TtrpC

  • GFP_TtrpC ran ligation PCR

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    10/1

  • GFP_TtrpC ran ligation PCR

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    10/2

  • GFP_TtrpC digested for ligation with pBARGPE1

  • Transformed pBAR_GFP_TtrpC into competent cells

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    10/3

  • pBAR_GFP_TtrpC containing cultures used in 2 in 1

  • pExtracted plasmid pBAR_GFP_TtrpC

  • pBAR_GFP_TtrpC digestion check(failure)

  • pBAR_GFP_TtrpC PCR check(failure)

  • Transformed pBAR_GFP_TtrpC into competent cells

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    10/4

  • pBAR_GFP_TtrpC containing cultures used in 2 in 1

  • pBAR_GFP_TtrpC plasmid extraction

  • pBAR_GFP_TtrpC digestion check

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