Difference between revisions of "Team:Michigan/Results"
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| − | <div class = "container"> <h1 style="text-align:center; font-size: 75px;"><font face= "Poiret One">Results</font></h1> | + | <div class = "container"> <h1 style="text-align:center; font-size: 75px;"><font face= "Poiret One">Results</font></h1><hr> |
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<h1 style="text-align:center; font-size: 50px;"><font face= "Poiret One">Creation of delta-M15 Mutant</h2><br> | <h1 style="text-align:center; font-size: 50px;"><font face= "Poiret One">Creation of delta-M15 Mutant</h2><br> | ||
| − | <p style="text-align:center; font-size:20px;"><font face="verdana"> Successfully deleted the alpha fragment in our Lacz plasmid | + | <p style="text-align:center; font-size:20px;"><font face="verdana"> Successfully deleted the alpha fragment in our Lacz plasmid, leaving only the omega fragment. The omega fragment complements the alpha fragment produced in the proximity-dependent ligation forming functional LacZ. This enzyme can break down X-Gal giving a colorimetric output. The plasmid is a very important part of our system, allowing alpha complementation and therefore producing a color visible to the naked eye. <br><br> |
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| + | Also, click <a href="https://static.igem.org/mediawiki/2016/7/77/Bench_to_Bedside_MSBT_PDF.pdf" target="_blank"><u>here</u></a> to see <a href="https://static.igem.org/mediawiki/2016/f/f8/Sequencing-Results-Using-T7-Primer-alignment_%281%29.pdf" target="_blank"><u>sequencing results</u></a>.</font><br><hr></p> | ||
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Revision as of 02:46, 20 October 2016
Results
Isolated LacZ from iGem part BBa_K564012
CAPTION HERE
Transferring LacZ into pET28
In order to have a control for our Cell Free expression system we cloned LacZ into the protein expression plasmid to compare LacZ expression to expression of our system with and without the presence of its target protein.
Creation of delta-M15 Mutant
Successfully deleted the alpha fragment in our Lacz plasmid, leaving only the omega fragment. The omega fragment complements the alpha fragment produced in the proximity-dependent ligation forming functional LacZ. This enzyme can break down X-Gal giving a colorimetric output. The plasmid is a very important part of our system, allowing alpha complementation and therefore producing a color visible to the naked eye.
Also, click here to see sequencing results.
Also, click here to see sequencing results.
