Difference between revisions of "Team:Lethbridge/Parts"

Revision as of 03:17, 20 October 2016

Lethbridge iGEM 2016

Our composite parts can be found here:

Composite Parts

BBa_K2109102 lambda c1 Nanobody GBP3
BBa_K2109103 Lambda c1 Nanobody scaffold
BBa_K2109104 Bacteria two hybrid selection system
BBa_K2109105 Bacteria two hybrid selection system with RNA polymerase alpha and pBAD
BBa_K2109106 RNA polymerase alpha
BBa_K2109107 RNA polymerase alpha GFP control
BBa_K2109109 U1A ribozyme control
BBa_K2109110 U1A-C75T ribozyme control

BBa_K2109111 - MS2 expression module containing a modifed MS2 protein to prevent dimerization to allow for higher yields during purification. Additionally a N-Terminal His tag was added and expression is modulated via a T7 promoter and strong RBS.
BBa_K2109108 - Mazf kill switch construct improved by adding RBS and testing the effects of evolutionary dirft.

@@ Line 269: / Line 269: @@
          <div class="col s12">
            <h3 class="thin white-text">Improved Parts</h3>
-           <h5 class="grey-text text-lighten-4">We improved a collection of parts this semester.</p>
+           <h5 class="grey-text text-lighten-4">We improved a collection of parts this semester.</h5>
              <ul>
                <li><a href="http://parts.igem.org/Part:BBa_K2109111">BBa_K2109111</a> - MS2 expression module containing a modifed MS2 protein to prevent dimerization to allow for higher yields during purification. Additionally a N-Terminal His tag was added and expression is modulated via a T7 promoter and strong RBS.</li>