Chris.isaac (Talk | contribs) |
Chris.isaac (Talk | contribs) |
||
Line 269: | Line 269: | ||
<div class="col s12"> | <div class="col s12"> | ||
<h3 class="thin white-text">Improved Parts</h3> | <h3 class="thin white-text">Improved Parts</h3> | ||
− | < | + | <p class="grey-text text-lighten-4">We improved a collection of parts this semester.</h5> |
<ul> | <ul> | ||
<li><a href="http://parts.igem.org/Part:BBa_K2109111">BBa_K2109111</a> - MS2 expression module containing a modifed MS2 protein to prevent dimerization to allow for higher yields during purification. Additionally a N-Terminal His tag was added and expression is modulated via a T7 promoter and strong RBS.</li> | <li><a href="http://parts.igem.org/Part:BBa_K2109111">BBa_K2109111</a> - MS2 expression module containing a modifed MS2 protein to prevent dimerization to allow for higher yields during purification. Additionally a N-Terminal His tag was added and expression is modulated via a T7 promoter and strong RBS.</li> |
Latest revision as of 03:19, 20 October 2016
Parts
Composite Parts
Our composite parts can be found here:
Composite Parts- BBa_K2109102 lambda c1 Nanobody GBP3
- BBa_K2109103 Lambda c1 Nanobody scaffold
- BBa_K2109104 Bacteria two hybrid selection system
- BBa_K2109105 Bacteria two hybrid selection system with RNA polymerase alpha and pBAD
- BBa_K2109106 RNA polymerase alpha
- BBa_K2109107 RNA polymerase alpha GFP control
- BBa_K2109109 U1A ribozyme control
- BBa_K2109110 U1A-C75T ribozyme control
Improved Parts
We improved a collection of parts this semester.
- BBa_K2109111 - MS2 expression module containing a modifed MS2 protein to prevent dimerization to allow for higher yields during purification. Additionally a N-Terminal His tag was added and expression is modulated via a T7 promoter and strong RBS.
- BBa_K2109108 - Mazf kill switch construct improved by adding RBS and testing the effects of evolutionary dirft.