Attributions
Project Design
The BeeT project consists of multiple parts, every student has their own part. We tried to integrate all the modelling with the wet lab. Our project has three main categories, sensing the
Varroa destructor, toxin production, and safety.
Wet lab
Lisa searched for the Bt toxin inside the Varroa,
Linea made a library of the toxin and
Jaccoline made vesicles.
Carina made the Varroa sensing system based on sensing mite poop and Vitamine B12.
Thomas made a quorum sensing system and a subpopulation system, based on a toxin/anti-toxin sytem.
Belwina used Cas9 to improve a biocontainment killswitch.
Marijn made the light kill switch in the lab, and
Tianhe took care of making a toggle switch and combining the light kill switch and sensing system together.
Modelling
Mario modelled the light kill switch which
Marijn was making in the lab.
Remco modelled the interaction between the Varroa and Bees and the spread of toxin through the hive.
Angelina modelled the quorum sensing subpopulation system and
Ronald worked on the metabolic modelling and a in vitro toxicity assay
Safety
As for the safety we want to implement a light kill switch in our end product. Also the Cas9 should prevent the bacteria from spreading through the environment.
Human Practice
Carina is our Captain Human Practice,
Carina has a Human Practice team with,
Linea and
Belwina, who help her with the Human Practice. As one of the first things that have been done for the Human practices is getting in contact with Beekeepers. Together with them we tried to think of a solution for the dying bees. We have a collaboration with
Synenergene where an application scenario was generated. A movie about synthetic biology has been made for the
RIVM.
Wiki Construction
Lisa is the Wiki Captain, together with
Ronald,
Remco and
Tianhe the design of the wiki was made. With the backbone of the
iGEM Wageningen_UR 2014 we made our team page.
Lisa made all the illustrations herself and together with the guys did most of the programming work.
Finances
With
Angelina as our Treasurer and together with
Jaccoline for the crowdfunding and
Mario for the plane tickets and accommodation. They searched for sponsors.
Other Team Roles
With
Thomas as our Team Captain the whole team functioned well. Off course he had much help from his secretary
Belwina.
Linea Captain Facebook, nothing is more important these days than presenting yourself at social media.
Marijn our lab safety manager took care of keeping the lab save and clean.
Supervisors
Christian Fleck, John van der Oost, Robert Smith, Kees van der Ark, Maarten Reijnders, Stamatios Damalas, Martha Viar Vazquez, Emma Keizer, Alex Kruis, Franklin Nobrega de Luzia.
Advisors
Bob Mulder