Project
This is our Modeling DesignBackground
Cancer is a large class of malignant diseases, 14 million new cases arising and causing over 8 million deaths per year. Which is the second most lethal disease in the world.[1]
Anti-microtubule agents , one of the most efficient medicines to treat many cancers including breast cancer、ovarian cancer and so on , are plant-derived chemicals that block cell mitosis by interfering with microtubule function . Microtubule is an important cellular structure composed of two proteins; α-tubulin and β-tubulin. They are hollow rod shaped structures that are required for cell division, among other cellular functions. [2] Microtubules are dynamic structures, which means that they are permanently in a state of assembly and disassembly. And the anti-microtubule agents can inhibit the dynamic balance of microtubule, leading to the termination of cell mitosis and inducing the apoptosis of tumor cells.
Anti-microtubule agents can be divided into two types : one inhibit assembly , such as vinca alkaloids、colchicine、podophyllotoxin and so on . The other inhibit disassembly , including taxanes、epothilones etc. Taxanes is the most famous among them.
Paclitaxel was discovered in 1962, isolated from the bark of the Pacific yew, Taxus brevifolia, thus its name “paclitaxel”. After only a brief period, the taxanes have demonstrated a unique ability to palliate the symptoms of many types of advanced cancers, including carcinoma of the ovary, lung, head and neck, bladder, and esophagus. Due to its good efficacy and slight side effect, the taxane class of anti-microtubule anticancer agents is perhaps the most important addition to the chemotherapeutic armamentarium against cancer over the past several decades.
Paclitaxel and other anti-microtubule medicines make a great success, which inspires many factories to extract these compounds, leading more and more famers to cultivate plants containing these compounds widely. Moreover, there are more and more scientists devote to study them. However, under this circumstance, many problems have alsobeen caused.
As to our project this year, we modified the homo sapiens tubulin alpha 1a , connected it with luciferase report gene’s N terminal or C terminal , and we put the modified α-tubulin and β-tubulin into E-coli to reduce them . Then we will get a kit containing the tubulins and buffer which has the appropriate condition verified by experiments. We call the kit “taxolight”, and it can do these things:
1. drug screen
Anti-cancer agents especially paclitaxel have showed their magnificent power in clinical application, but also are a little unsatisfactory. We still need to look for new drugs that more effective.
The existing method to screen anti-microtubule agents needs purifying tubulins of mammalian brains. It relies on the features of tubulins that the solution turbid will increase when then polymerize in vitro under 37℃ centigrade. So using this method, we can get a polymerization curve shaped sigmoid formed by the liquid OD value to the soaking time, correspondingly, we can also get a de-polymerization curve when putting the tubulins into ice. When adding different anti-microtubule agents, polymerization of "S" type curve or pour de-polymerization of "S" type curve has different effects, and we can determine the role of the drug according to the change of curve.
Our project provides a new idea on the drug screen of anti-microtubule agents: using our kit, add the two kinds of quantitative modified α-tubulins withβ-tubulins and buffer, and put the quantitative sample, and then measure its fluorescence intensity. Paclitaxel can be set as a standard, and we can compare the new medicine with paclitaxel by comparing the fluorescence intensity. In this way, the primary invitro screening of compounds that can influence microtubules can be carried out using our kit, to further research and development of new anti-microtubule agents.
