Team:Wageningen UR/Notebook/ProteinEngineering
May
Week 1
Setting up the lab, preparing media, preparing electrocompetent cells, primer design etc.
Week 2
PCR of Cry3Aa from Bacillus thuringiensis var. tenebrionis and Cry1Ab from Bacillus thuringiensis Berliner 1915. Success with PCR of Cry3Aa, but not Cry1Ab. Creation of vector pBbA7c-Cry3Aa.
Week 3
I tried to transform the created vector pBbA7c-Cry3Aa into Lemo21. No success.
Week 4
A lot of time spent on trying to clone the vector pBbA7c/Cry3Aa into Lemo21. Inconclusive results.
June
Week 5-8
No chance of working in the lab due to moving of the lab.
July
Week 9
A fresh start with a lot of work for setting up the lab again. I learned the basics of phage work in this and the next week.
Week 10
Continuation of learning how to work with bacteriophages. Mite feeding experiments with fluorophores: Mites do take up fluorophores with their food! But only a small amount of them (~16 %).
Week 11
Feeding experiment with mealworms. Mealworms are easy to feed, easy to dissect and easy to keep. They take up fluorescine when fed with carrots dipped in it.
Week 12
vacation
August
Week 13
Week 18-19
Week 20
September
Week 21
Week 22
