Team:Glasgow/Attributions
Katy Baker: Worked on transforming S. thermophilus with BioBrick constructs. Developed Miniprep protocol for purifying plasmid DNA from S. thermophilus. Demonstrated at Glasgow Science Centre. Social media boss. Pinja Hartikainen: Created crtEBIY BioBrick compatible construct through a series of gene ligations. Presented at London teams meetup at Westminster. Catriona Watson: Designed RNA extraction experiments for transcriptomics collaboration with Glasgow Polyomics. Demonstrated at Glasgow Science Centre. Created the final design for our mascot, Beta. Liam Pollock: Created crtEBIY BioBrick compatible construct. Quantified levels of beta-carotene in E. coli with various native S. thermophilus promoters. Demonstrated at Glasgow Science Centre. Matej Vucak: Created and quantified BioBrick constructs for use with S. thermophilus. Carried out Interlab measurements and submission. Deanna van Spall: Isolated the antitoxin Epsilon for potential self-inactivation mechanism. Demonstrated at Glasgow Science Centre. Lindsey McKerrell: Designed and created an accessible piece of hardware for solar pasteurisation. Simone Marcigaglia: Designed and created an accessible piece of hardware for solar pasteurisation. Demonstrated at Glasgow Science Centre. Fergus O’Brien: Designed and created an accessible piece of hardware for solar pasteurisation. Presented at London teams meetup at Westminster. Demonstrated at Glasgow Science Centre. Oliver Hawes: Our tech guy. Wiki coding and design.
Supervisors/Advisors Dr Julien Reboud (Lord Kelvin Adam Smith Fellow in Synthetic Biology, Biomedical Engineering, University of Glasgow) Supervisor: Our primary advisor - provided plate reader for fluorescence measurements, gave us useful presentation feedback, and bought us pizza that one time. Dr Sean Colloms (Lecturer in Synthetic Biology, Institute of Molecular Cell and Systems Biology, University of Glasgow) Supervisor: Our secondary supervisor - Provided advice about molecular biology. James Provan - Advisor: Demonstrated lab techniques (multiple times). Showed incredible patience. Mhairi Davidson - Advisor: Provided support with Exeter collaboration and general lab techniques. Dr Steph Holt: Helped with electroporating S. thermophilus when things were looking impossible Engineering dudes Ewan Russell: for 3D printing our magnificent valve. Julien’s guy: for 3D printing our more magnificent valve 2.0. Rankine Building Engineering Department: Thanks for the electronics! General Support Everyone in the Bower Building, University of Glasgow, for their support over the summer. Thanks to the Scottish weather for providing us with one day of sun so we could test our initial solar concentrator.
Human Practice Support Dr John Walls (Research Associate, School of Interdisciplinary Studies, University of Glasgow) Advised on public interest and social sciences Jamie Gallagher (Public Engagement Officer, Research Strategy and Innovation Office, University of Glasgow) Outreach help and advice Melanie Jimenez: Thanks for our super cute video for the Science centre. Glasgow Science Centre, for allowing us to participate in the Meet the Experts event.
Project Support
Glasgow Polyomics - Performed ten RNAseq experiments in order to distinguish differential gene expression in S. thermophilus based on casein exposure. Maud Flechard and Pascal Hols: Gifted us with S. thermophilus LMD-9 strain, multiple plasmids, and protocols for working with S. thermophilus iGEM Team Exeter - for providing us with an alternative Kill Switch for our yogurt. Thanks to Zuhair Elgaid, our summer intern, for helping with initial research.
