Component | Dosage |
---|---|
10×N6macroelement solution | 50 mL |
1000×B5microelement solution | 0.5 mL |
100×B5vitamin solution | 5 mL |
100×Ferric salt solution | 5 mL |
0.5 mg/mL 2,4-D | 3 mL |
Casein enzymatic hydrolysate | 150 mg |
L-Proline | 250 mg |
L-Glutamine | 250 mg |
Sucrose | 15 g |
Add distilled water until the medium volume reach 500 mL | |
Blend withmagnetic stirrer | |
Adjust medium pH to 5.8 with 1 mol/L NaOH | |
Phytagel | 1.5 g |