Team:Wageningen UR/Notebook
Find the documentation of our research here.
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Protein Engineering
We wanted to engineer Cry3Aa to become toxic to Varroa destructor.
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In Vitro Assay
To provide an alternative for tests on live Varroa mites, we designed an assay that uses brush border membrane vesicles.
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Varroa isolates
We tried to find our own Varroa entomopathogen.
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Riboswitches
To detect the presence of Varroa mites in the beehive, we made a B12 and a guanine riboswitch.
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Quorum Sensing
A quorum sensing system was characterized to regulate toxin production in BeeT.
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Optogenetic Kill Switch
The light-induced kill switch was one of our biocontainment methods.
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Modelling
How we modelled .
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Chitinases
As a back-up, we wanted to use chitinases which are more harmful to Varroa destructor than honeybees.
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Cas9-based Kill Switch
The Cas9-based kill switch was one of our biocontainment methods.
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Toxin Scanner
We made a software tool for finding new Cry proteins toxic to Varroa destructor.
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Metabolic Modelling
We wanted to use a metabolic model to see the effect of our BeeT distribution method.
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Testing E. coli survival
Following the results of the Metabolic Model, we needed more data.
