Team:Wageningen UR/Parts

Wageningen UR iGEM 2016

 

BioBrick number BioBrick name Designer
BBa_K1913000 chiA for Varroa destructor Lisa
BBa_K1913001 chiB for Varroa destructor Lisa
BBa_K1913002 chiA device regulated by pBAD Lisa
BBa_K1913003 chiB device regulated by pBAD Lisa
BBa_K1913005 lux quorum sensing system + GFP reporter Thomas
BBa_K1913006 434- and lambda cI balance operon + mRFP reporter Thomas
BBa_K1913007 434- and lambda cI operon for tuning protein balance Thomas
BBa_K19130014 3-oxo-hexanoyl-HSL GFP reporter Thomas
BBa_K19130016 434- and lambda cI balance RFP reporter Thomas
BBa_K1913008 vitamin b12 riboswitch Carina
BBa_K1913009 Guanine riboswitch Carina
BBa_K19130010 tetR QPI + mRFP Carina
BBa_K19130011 Vitamin b12 riboswitch + mRFP Carina
BBa_K19130012 Guanine riboswitch + guanine riboswitch Carina
BBa_K19130019 Guanine riboswitch BS-yxjA Tianhe
BBa_K19130020 mRFP with degredation tag Tianhe
BBa_K19130021 sGFP with defredation tag Tianhe
BBa_K19130022 Artificial FixK2 promoter with lac operon O1, O3 consitutive promoter Tianhe
BBa_K19130023 Artificial FixK2 promoter with lac operon O1, O3 ompR Tianhe
BBa_K19130024 Artificial FixK2 promoter with two tetO operons Tianhe
BBa_K19130025 Natural FixK2 promoter with lac operons O1, O3 Tianhe
BBa_K19130026 Natural FixK2 promoter with two tetO operons Tianhe
BBa_K19130027 Wild type plac-FixK2 hybrid promoter with mRFP Tianhe
BBa_K19130028 Wild type ptet-FixK2 hybrid promoter with mRFP Tianhe
BBa_K19130029 Synthetic plac-FixK2 hybrid promoter +RBS with mRFP Tianhe
BBa_K19130030 Synthetic plac-FixK2 hybrid promoter+RBS with mRFP Tianhe
BBa_K19130031 Syntheitc ptet-FixK2 hybrid promoter+RBS with mRFP Tianhe
BBa_K19130032 Toggle Switch device Tianhe
BBa_K19130033 Toggle Switch device Tianhe

Besides, we submitted one part that cannot be classified as a biobrick:

BBa_K1913015 Cry3Aa with araC/pBAD Jaccoline/Linea

When we started making the constructs for the Cas9 kill switch, two approaches were taken: one was taking the Cas9 that is available in the iGEM registry (BBa_K1218011) as a starting point for making mutations and expressing Cas9. The other approach was starting with pdCas9 (Addgene plasmid # 46569). While cloning, it proved to be difficult to transfer BBa_K1218011 to another backbone (see also the notebook). Furthermore, when cultures transformed with BBa_K1218011 were checked for Cas9 expression with SDS-PAGE, no convincing Cas9 band could be observed. Because of time limitations we decided to continue working with the Addgene construct for making the mutations, and chose an established system for protein expression. For that reason, no Cas9-biobricks were submitted. Furthermore, the pEVOL construct containing an aminoacyl-synthetase and a tRNA for introducing BipA in response to the TAG stopcodon were isolated from a strain kindly received from George Church (described in Mandel et al., 2015). The MTA that was signed to receive the strain does not allow for redistribution.

BioBrick 1

YOUR TEXT HERE
Link to favourite biobrick should link to Wageningen_UR/Basic_Part
Link to set of favourite biobricks should link to Wageningen_UR/Part_Collection

BioBrick 2

BioBrick 3