Team:Wageningen UR/Parts
| BioBrick number | BioBrick name | Designer |
|---|---|---|
| BBa_K1913000 | chiA for Varroa destructor | Lisa |
| BBa_K1913001 | chiB for Varroa destructor | Lisa |
| BBa_K1913002 | chiA device regulated by pBAD | Lisa |
| BBa_K1913003 | chiB device regulated by pBAD | Lisa |
| BBa_K1913005 | lux quorum sensing system + GFP reporter | Thomas |
| BBa_K1913006 | 434- and lambda cI balance operon + mRFP reporter | Thomas |
| BBa_K1913007 | 434- and lambda cI operon for tuning protein balance | Thomas |
| BBa_K19130014 | 3-oxo-hexanoyl-HSL GFP reporter | Thomas |
| BBa_K19130016 | 434- and lambda cI balance RFP reporter | Thomas |
| BBa_K1913008 | vitamin b12 riboswitch | Carina |
| BBa_K1913009 | Guanine riboswitch | Carina |
| BBa_K19130010 | tetR QPI + mRFP | Carina |
| BBa_K19130011 | Vitamin b12 riboswitch + mRFP | Carina |
| BBa_K19130012 | Guanine riboswitch + guanine riboswitch | Carina |
| BBa_K19130019 | Guanine riboswitch BS-yxjA | Tianhe |
| BBa_K19130020 | mRFP with degredation tag | Tianhe |
| BBa_K19130021 | sGFP with defredation tag | Tianhe |
| BBa_K19130022 | Artificial FixK2 promoter with lac operon O1, O3 consitutive promoter | Tianhe |
| BBa_K19130023 | Artificial FixK2 promoter with lac operon O1, O3 ompR | Tianhe |
| BBa_K19130024 | Artificial FixK2 promoter with two tetO operons | Tianhe |
| BBa_K19130025 | Natural FixK2 promoter with lac operons O1, O3 | Tianhe |
| BBa_K19130026 | Natural FixK2 promoter with two tetO operons | Tianhe |
| BBa_K19130027 | Wild type plac-FixK2 hybrid promoter with mRFP | Tianhe |
| BBa_K19130028 | Wild type ptet-FixK2 hybrid promoter with mRFP | Tianhe |
| BBa_K19130029 | Synthetic plac-FixK2 hybrid promoter +RBS with mRFP | Tianhe |
| BBa_K19130030 | Synthetic plac-FixK2 hybrid promoter+RBS with mRFP | Tianhe |
| BBa_K19130031 | Syntheitc ptet-FixK2 hybrid promoter+RBS with mRFP | Tianhe |
| BBa_K19130032 | Toggle Switch device | Tianhe |
| BBa_K19130033 | Toggle Switch device | Tianhe |
Besides, we submitted one part that cannot be classified as a biobrick:
| BBa_K1913015 | Cry3Aa with araC/pBAD | Jaccoline/Linea |
When we started making the constructs for the Cas9 kill switch, two approaches were taken: one was taking the Cas9 that is available in the iGEM registry (BBa_K1218011) as a starting point for making mutations and expressing Cas9. The other approach was starting with pdCas9 (Addgene plasmid # 46569). While cloning, it proved to be difficult to transfer BBa_K1218011 to another backbone (see also the notebook). Furthermore, when cultures transformed with BBa_K1218011 were checked for Cas9 expression with SDS-PAGE, no convincing Cas9 band could be observed. Because of time limitations we decided to continue working with the Addgene construct for making the mutations, and chose an established system for protein expression. For that reason, no Cas9-biobricks were submitted. Furthermore, the pEVOL construct containing an aminoacyl-synthetase and a tRNA for introducing BipA in response to the TAG stopcodon were isolated from a strain kindly received from George Church (described in Mandel et al., 2015). The MTA that was signed to receive the strain does not allow for redistribution.
BioBrick 1
YOUR TEXT HERE
Link to favourite biobrick should link to Wageningen_UR/Basic_Part
Link to set of favourite biobricks should link to Wageningen_UR/Part_Collection
BioBrick 2
BioBrick 3
