7/31: Transformed C120_minimal PgpdA into competent cells
7/31: C120_minimal PgpdA containing cells used in 2 in 1
8/1: C120_minimal PgpdA extracted from transformed cells
8/1: C120_minimal PgpdA checked with restriction digestion
8/2: C120_minimal PgpdA amplified with plasmid PCR
8/3: C120_minimal PgpdA digested for ligase with RFP
8/3: C120_minimal PgpdA_RFP transformed into competent cell
8/4: C120_minimal PgpdA_RFP extracted from transformed cells
8/4: C120_minimal PgpdA_RFP amplified with plasmid PCR and checked with restriction digestion
8/6: C120_minimal PgpdA_RFP plasmid stored and transformed cultures cryopreserved
8/14: C120_minimal PgpdA_RFP digested for ligation with TtrpC
8/15: C120_minimal PgpdA_RFP_TtrpC transformed into competent cell
8/17: C120_minimal PgpdA_RFP_TtrpC extracted and checked with restriction digestion
8/17: C120_minimal PgpdA_RFP_TtrpC amplified with plasmid PCR
8/18: C120_minimal PgpdA_RFP_TtrpC plasmid stored and transformed bacterial cultures cryopreserved
