Team:Waterloo/Parts

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• Description
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• Premature Stop Codon
• Promoter Selection
• Gene Retention Model
• Aggregation Model
• Aggregation Model

• Proof Of Concept
• Gene Retention
• Promoter Analysis

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Our System

Hsp104-NSC

Hsp104-SC1

Hsp104-SC2

Biobrick

ADH1-GFP

Gal1-GFP

Cup1-GFP

Gal1-CFP NSC

Gal1-CFP SC1

Gal1-CFP SC2

Promoter Testing

Hsp104-Gal1

Hsp104-Cup1

pXP218-ADH1

pXP218-Gal1

pXP218-Cup1

Hsp104-NSC

This construct is a control to test the metabolic load of the Hsp104-CFP fusion protein and as a baseline against which we compare the change in [PSI+]/[psi-] state. We synthesized a fragment to clone into the [Hsp-PRS315]. This plasmid will be referred to as the Hsp104 plasmid and is used in several of our experiments.