Team:HokkaidoU Japan/Overview

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Team:HokkaidoU Japan

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• Top
• Project
  • Overview
    • Project Description
    • Background
    • Proof of concept
    • Modeling
    • Achievements
  • MULTIMERIZATION
    • Overview
    • Methods
    • Results
    • Conclusion
  • FUTURE WORK
    • Circularization
    • Kill Switch
    • Transport
    • Aggregation
  • Extra Materials
    • Parts
    • Notebook
    • Protocols
    • Safety
• Human practices
• Collaborations
• Team
  • Attribution
  • Member

We considered possible application of self-assembling peptides (SAPs). SAPs have a self-assembling ability because they are amphiphilic peptides and contain hydrophobic and hydrophilic residues. These electric charged amino acids interact with one another to form spontaneously antiparallel β-sheet in a physiochemical environmental condition.

In this projects, we used one of SAPs, RADA-16-I(RADARADARADARADA) and P₁₁-4 (QQRFEWEFEQQ).

RADA-16-I has been established for 3-D culture of neural stem cells (NSCs) by creating nanostructures. P₁₁-4 has been designed to form fibers at low pH and have a cytocompatibility.

Recently, SAPs have been regarded as good biological material. We considered new application methods of SAPs through discussions. Finally, we decided to make a platform of technology for constructing multi-enzyme-complex by using Self-Assembling Regions (SARs) and they are covalently linked each other through disulfide bonds. They could be assemble multiple enzymes ring (Self-AssembRing).

In the future, we will construct tool for making subunits of artificial multi-enzyme-complex by using this technology.

Recently, self-assembling peptides (SAPs) have been remarked as a biological nanostructures and hydrogel because SAPs are fit to biogenic and don’t have cytotoxicity. SAPs such as a scaffold for cell culturing and drug delivering in tissue engineering and medical technology.

Bronze

We completed the team registration.

We wrote team wiki.

Poster and a talk for the iGEM Jamboree are ready.

We described all attributions clearly.

We created and documented Parts pages.

We submitted DNA samples of our new BioBrick Parts.

We filled out Safety forms.

We filled out Judging form.

Silver

We validated our new BioBrick parts.

We collaborated with other iGEM teams (Gifu, Kyoto, Nagahama, UT-tokyo, METU HS Ankara, Heidelberg)

We considered that prevalence of synthetic biology

Gold

We improved a previous part.

We demonstrated a functional proof of concept of our project.