Team:Michigan/Basic Part

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Basic Parts

BBa_K2105000

The lacZ delta M15 mutation encodes a form of beta-galactosidase lacking residues 11-41. This mutation causes it to be inactive until complemented by the lacZ alpha fragment. The lacZ delta M15 mutant is constitutively expressed in DH5 alpha cells. When the delta M15 mutant and the alpha fragment undergo alpha complementation, they are able to cleave the substrate X-gal to produce a blue product.

Registry of Standard Biological Parts Entry

BBa_E0033

To greatly reduce the number of bases, this is only a portion of the LacZ gene. It can be used as a detection system with N-terminal deletion mutants of lacZ. Combination of lacZ-alpha with such a deletion mutant protein restores enzyme activity that can be assayed colorimetrically or more sensitively with chemiluminescent substrates.

Registry of Standard Biological Parts Entry