Team:UBonn HBRS/Description/Notebook/2016

Lab Notebook 2016

10KW (7.3-13.3)

transformation of genes bought at genescript: XynA, XynB, LipA, EstC2, CpCel9, CpCel5c, EngB, CelA, BsCel5, Bpul and Linkers: nprb, SacB inoculation of liquid cultures and restriction Analysi of linkers (SacB and nprb).

repetition of the transformation for XynB, EngB and linkers. Subsequently inoculation and miniprep of the samples. Preparation of glycerol stock.

restriction analysis of the genes.


11KW (14.3-20.3)

Repetion of restriction analysis from the genes KW10 and gel clean ups of the linkers.

Deinking:

  • Measurement of inkjet absorbance spectrum
  • filtration-based deinking setup


12KW (21.3-27.3)

repetition of restriction analysis of all genes and gel clean ups of the genes.

Deinking: inkjet ink absorbance spectrum and pulp preparation


13KW (28.3-3.4)

Restrictions and gel clean ups of all genes genes for a new gel clean up, because the ones of KW13 did not works. Ligation of genes from the gel clean ups into C3 and inoculation of cultures and minipreps. (1.04.16 :) -> successful ligation of

  • XynA
  • CelA
  • LipA
  • Bpul
  • CpCel5c
  • BsCel5
  • EstC2
  • EngB in C3
  • nprb + CpCel9
  • nprb + BsCel5 in C3

Deinking: establishing the set up and starting experiments with different conc of NaOH and Na2SiO3


14KW (4.4-10.4)

Deinking: Experiments with different concentrations of surfactant oleic acid


15KW (11.4-17.4)

Deinking: experiments with different incubation time, vortexing time, temperature


16KW (18.4-24.4)

Deinking: tests with solvents to dissolve ink and fibers


17KW (25.4-1.5)

Deinking: grayscale tests, solvent tests


18KW (2.5-8.5)

Deinking: pulp preparation


19KW (9.5-15.5)

Deinking: establishing water and standard chemical controls


20KW (16.5-22.5)

Deinking: establishing water and standard chemical controls


21KW (23.5-29.5)

Restriction analysis of genes from the 13KW and mini prep from samples of the 13KW. (28.05.16) successful ligation of:

  • nprb and sacB in C3
  • nprb + EstC2 in C3

Deinking: establishing oleic acid and oleic acid + citrate buffer controls


22KW (30.5-5.6)

Restriction analysis of samples of the 21KW Deinking: discovery: paper discs with CB turn red, tests to find the reason


23KW (6.6-12.6)

Inoculation of all genes from glycerol stocks and miniprep. Restriction analysis and gel clean up of the genes, ligation of the missing genes with C3. Deinking: tests with oleic acid and oleic acid + cb controls


24KW (13.6-19.6)

Restriction of bbMCS and gel clean ups. Ligations of genes in C5 and C3 Ligation of genes and tags in C5. Minipreps of all of the samples. Deinking: tests with shaking during incubation and different incubation time


25KW (20.6-26.6)

Restriction analysis of the constructs Repetition of ligation of genes in C5 and transformation and preps. Deinking: rotation evaporator concentrating ink


26KW (27.6 - 3.7)

Restriction analysis of the mini preps.


28KW (11.7-17.7)

Repetion of Ligations of genes and C5 Deinking: flotation-based deinking, pulp preparation

29KW (18.7-24.7)

Deinking: separation of ink from fibers in organic phase of big scale deinking, flotation-based deinking, foam collection, defoaming


30KW (25.7-31.7)

Deinking: tests with toluene flotation-based deinking


31KW (1.8-7.8)

Ligation of Fragment synthesized at IDT and C3 Ligation of genes in C3 and genes + tags + C5 transformation, and inoculation of liquid cultures of the above.


32KW (8.8 - 14.8)

Ligation of IDT and C3 Transformation and inoculation. Minipreps of samples and restriction analysis

Ligations of genes plus tags in bb_MCS Transformation of these samples


33KW (15.8 - 21.8)

Inoculation and miniprep Repetion of ligation of IDT and C3 Repetion of ligation of bb + nprb + genes Midi prep of SacB, bb and nprb Ligation of Genes + C5 and genes plus tags in C5. Transformation and inoculation of the samples. mini preps Deinking: creation of flat paper-like discs


34KW (22.8 - 28.8)

Restrictions of mini prep and repetition of mini prep

Deinking: grayscale tests with officer scanner and odisey scanner


35KW (29.8 - 4.9)

36KW (5.8 - 11.8)

Deinking: tests of water controls


37KW (12.9 - 18.9)

Deinking: scanning tests, different conditions tests


38KW (19.9 - 25.9)

39KW (26.8 - 2.10)

Deinking: establishing water and standard controls into setup with kitchen mixer


40KW (3.10 - 9.10)

Deinking: xylanase started to test + cb


41KW (10.10 - 16.10)

Deinking: xyl tests + cb


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