Team:NYMU-Taipei/Notebook-Lab Book-C120 minPgpdA RFP TtrpC
Transformed C120_minimal PgpdA into competent cells
C120_minimal PgpdA containing cells used in 2 in 1
C120_minimal PgpdA extracted from transformed cells
C120_minimal PgpdA checked with restriction digestion
C120_minimal PgpdA amplified with plasmid PCR
C120_minimal PgpdA digested for ligase with RFP
C120_minimal PgpdA_RFP transformed into competent cell
C120_minimal PgpdA_RFP extracted from transformed cells
C120_minimal PgpdA_RFP amplified with plasmid PCR and checked with restriction digestion
C120_minimal PgpdA_RFP plasmid stored and transformed cultures cryopreserved
C120_minimal PgpdA_RFP digested for ligation with TtrpC
C120_minimal PgpdA_RFP_TtrpC transformed into competent cell
C120_minimal PgpdA_RFP_TtrpC extracted and checked with restriction digestion
C120_minimal PgpdA_RFP_TtrpC amplified with plasmid PCR
C120_minimal PgpdA_RFP_TtrpC plasmid stored and transformed bacterial cultures cryopreserved
