Team:NYMU-Taipei/Notebook-Lab Book-C120 minPgpdA RFP TtrpC

C120 minPgpdA RFP TtrpC

C120 minPgpdA RFP TtrpCLab note


  • C120_empty

  • minimal-PgpdA

  • RFP

  • TtrpC


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    7/31

  • Transformed C120_minimal PgpdA into competent cells

  • C120_minimal PgpdA containing cells used in 2 in 1

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    8/1

  • C120_minimal PgpdA extracted from transformed cells

  • C120_minimal PgpdA checked with restriction digestion

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    8/2

  • C120_minimal PgpdA amplified with plasmid PCR

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    8/3

  • C120_minimal PgpdA digested for ligase with RFP

  • C120_minimal PgpdA_RFP transformed into competent cell

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    8/4

  • C120_minimal PgpdA_RFP extracted from transformed cells

  • C120_minimal PgpdA_RFP amplified with plasmid PCR and checked with restriction digestion

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    8/6

  • C120_minimal PgpdA_RFP plasmid stored and transformed cultures cryopreserved

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    8/14

  • C120_minimal PgpdA_RFP digested for ligation with TtrpC

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    8/15

  • C120_minimal PgpdA_RFP_TtrpC transformed into competent cell

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    8/17

  • C120_minimal PgpdA_RFP_TtrpC extracted and checked with restriction digestion

  • C120_minimal PgpdA_RFP_TtrpC amplified with plasmid PCR

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    8/18

  • C120_minimal PgpdA_RFP_TtrpC plasmid stored and transformed bacterial cultures cryopreserved

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