Team:Lethbridge/Parts

Lethbridge iGEM 2016

Parts

I really need to link these soon.

Composite Parts

Our composite parts can be found here:

Composite Parts
  • BBa_K2109102 lambda c1 Nanobody GBP3
  • BBa_K2109103 Lambda c1 Nanobody scaffold
  • BBa_K2109104 Bacteria two hybrid selection system<
  • BBa_K2109105 Bacteria two hybrid selection system with RNA polymerase alpha and pBAD<
  • BBa_K2109106 RNA polymerase alpha<
  • BBa_K2109107 RNA polymerase alpha GFP control<
  • BBa_K2109109 U1A ribozyme control<
  • BBa_K2109110 U1A-C75T ribozyme control<

Improved Parts

We improved a collection of parts this semester.

  • BBa_K2109111 - MS2 expression module containing a modifed MS2 protein to prevent dimerization to allow for higher yields during purification. Additionally a N-Terminal His tag was added and expression is modulated via a T7 promoter and strong RBS.<
  • BBa_K2109108 - Mazf kill switch construct improved by adding RBS and testing the effects of evolutionary dirft.<