Difference between revisions of "Team:HokkaidoU Japan/Proof"
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We conducted SDS-PAGE with | We conducted SDS-PAGE with | ||
| − | (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on （pSB1C3）of E. | + | (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on （pSB1C3）of <span style="font-style: italic">E.coli</span>（DH5α）. |
At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because | At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because | ||
Revision as of 11:43, 18 October 2016
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Team:HokkaidoU Japan
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We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on （pSB1C3）of E.coli（DH5α）. At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on （pSB1C3）of E.coli（DH5α）. At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
| Materials | Volume |
|---|---|
| 30％ Acrilmid | 5 mL |
| Tris-Buffer | 2.5 mL |
| SDS | 100 µL |
| APS | 100 µL |
| TEMED | 5 µL |
| DW | 2.295 ｍL |
| Total | 10 ｍL |
