Line 75: | Line 75: | ||
<br> | <br> | ||
− | The following | + | The following Table. 2 is about preparing the SDS-PAGE. |
− | + | <center> | |
<table style="border-style: none"> | <table style="border-style: none"> | ||
<tr align="center"> | <tr align="center"> | ||
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</table> | </table> | ||
</center> | </center> | ||
+ | <br clear="all"> | ||
<br> | <br> | ||
− | + | After we finished cultivating samples, | |
+ | we took 100 µL out of each samples and made the following operation. | ||
<br> | <br> | ||
+ | |||
<ol> | <ol> | ||
<li>Centrifuge with 13000rpm at 24°C for 2min</li> | <li>Centrifuge with 13000rpm at 24°C for 2min</li> | ||
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<li>Keep at 100°C for 5 min</li> | <li>Keep at 100°C for 5 min</li> | ||
<li>Put on the ice</li> | <li>Put on the ice</li> | ||
− | <li>Apply 10 µ to SDS</li> | + | <li>Apply 10 µL to SDS</li> |
<li>Run electrophoresis for 1.5 h</li> | <li>Run electrophoresis for 1.5 h</li> | ||
<li>Wash out with MiliQ</li> | <li>Wash out with MiliQ</li> |
Revision as of 12:56, 18 October 2016
Team:HokkaidoU Japan
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We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on (pSB1C3)of E.coli(DH5α). At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
The following Table. 2 is about preparing the SDS-PAGE.
We conducted SDS-PAGE with (BBa_K2015012+BBa_K2015008/ BBa_K2015012+BBa_K2015009) on (pSB1C3)of E.coli(DH5α). At first, we made the Gel for SDS-PAGE, with the following the Table1. What was the most important was to add TEMED ffinally because
Table. 1. Gel assay |
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The following Table. 2 is about preparing the SDS-PAGE.
Table. 2. Experimental condition |
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After we finished cultivating samples, we took 100 µL out of each samples and made the following operation.
The fig. 1 shows the result of SDS-PAGE. |