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− | <h3> | + | <h3>BBa_I746908 characterization</h3> |
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− | + | E. coli BL21 (DE3) was transformed with BBa_I746908 and a single colony was inoculated into LB broth (with the appropriate concentration of antibiotic) primary that was grown overnight (37 celcius, 180 rpm) and then 1% of the primary was inoculated into 8 x 250mL conical flasks, 2 each with the following: | |
+ | <br> | ||
+ | 1. 100mL LB broth (blank) <br> | ||
+ | 2. 100mL LB broth + 0.2% glucose <br> | ||
+ | 3. 100mL LB broth + 0.2% arabinose <br> | ||
+ | 4. 100mL LB broth + 0.2% arabinose + 0.2% glucose <br> | ||
+ | 0.5mL samples were collected every hour in microfuge tubes and stored at 4C and the flasks were incubated at 37C, 180rpm. OD600 and fluorescence values of the samples were recorded. <br> | ||
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+ | <h3>BBa_K1352000 characterization</h3> | ||
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+ | <br>1. Four 25 ml of LB media in conical flasks were inoculated from an overnight primary culture of E. coli BL21 (DE3) transformed with appropriate part (BBa_K1072000 for lux part and BBa_R0040 for negative control) in pSB1C3 (1 % inoculation, 37 degree celcius, 180 RPM).<br> | ||
+ | The cultures were grown in an orbit shaker incubator at 37 degree celcius, 180 RPM.<br> | ||
+ | 200 uL aliquots were taken from each conical flask every 2 hours and stored at 4 degree celcius.<br> | ||
+ | GFPmut3 fluorescence (Ex: 501 nm, Em: 511 nm) and OD600 of the samples were recorded on a plate reader (Tecan M2000 InfinitePro). | ||
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+ | For results of these experiments, please refer to <a href="https://2016.igem.org/Team:IISc_Bangalore/Results">Results</a> page. | ||
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Revision as of 21:41, 19 October 2016