Favorite Basic Part: BBa_k2139003
BBa_K2139003 is comprised of the coding region of E1. E1 was used as a fusion protein within the middle of Caulobacter cresentus’s RSA-A surface layer protein. We were able to show expression on the surface of C. crescentus and functionally characterize that it maintained functionality. You can see our data here. The part originated from Acidothermus cellulolyticus and was codon optimized for expression in C. crescentus. Our biobrick contains only the catalytic domain of the full protein.
Despite this being UBC iGEM's favorite part, we were additionaly able to characterize 3 other parts: BBa_K2139001, BBa_K2139002, BBa_K2139004. Check out each part and their data on the Cellulases and Laccases section of our project.
References: Linger, Jeffrey G., William S. Adney, and Al Darzins. "Heterologous Expression and Extracellular Secretion of Cellulolytic Enzymes by Zymomonas Mobilis." Applied and Environmental Microbiology, vol. 76, no. 19, 2010., pp. 6360-6369doi:10.1128/AEM.00230-10.
Check out other parts of our project below!