Improving the registry one BioBrick at a time
The WashU-PSU iGEM team tested 2 genes of interest in order to overproduce ATP (pck and pgk) and 3 genes of interest in order to overproduce electron donors (fldA, petF, and pfo). This resulted in 6 total constructs: two for ATP and four for electron donors. From these constructs, pck produced the most ATP and petF (and petF-pfo) successfully produced reduced eletron donors. Because of this, we chose to turn these genes into BioBricks. Additionally, we decided to turn the inducible promoter from our electron donor constructs, pTet, into a BioBrick, which included its repressor protein, TetR. Previous parts had only the pTet promoter region, making it a constitutive promoter. Our part allows for pTet to be an inducible promoter, controlled by the addition of aTc. Finally, we created a composite part with petF and TetR-pTet, which was the relevant gene sequence in the petF construct.
Additionally, we characterized an HSP BioBrick that had no previous data for our Gold medal requirement.
|Part Name||Part Number||Part Function||In Our Project|
|pck||BBa_K2135000||Codes for PCK||Used to overproduce intracellular ATP|
|petF||BBa_K2135001||Codes for ferredoxin||Used to overproduce reduced electron donors|
|TetR-pTet||BBa_K2135002||Inducible promoter with addition of aTc||Allowed control for genes of interest|
|TetR-pTet-petF||BBa_K2135003||Ferredoxin production controlled by inducible promoter||Allowed control on overproducing reduced electron donors|
|Part Name||Part Number||Part Function|
|HSP Promoter||BBa_K873002||Heat activated promoter|