Team:WashU StLouis/Parts


Improving the registry one BioBrick at a time

The WashU-PSU iGEM team tested 2 genes of interest in order to overproduce ATP (pck and pgk) and 3 genes of interest in order to overproduce electron donors (fldA, petF, and pfo). This resulted in 6 total constructs: two for ATP and four for electron donors. From these constructs, pck produced the most ATP and petF (and petF-pfo) successfully produced reduced eletron donors. Because of this, we chose to turn these genes into BioBricks. Additionally, we decided to turn the inducible promoter from our electron donor constructs, pTet, into a BioBrick, which included its repressor protein, TetR. Previous parts had only the pTet promoter region, making it a constitutive promoter. Our part allows for pTet to be an inducible promoter, controlled by the addition of aTc. Finally, we created a composite part with petF and TetR-pTet, which was the relevant gene sequence in the petF construct.

Additionally, we characterized an HSP BioBrick that had no previous data for our Gold medal requirement.

Click on the part in the table below to view the registry entry, or view a summary of our Basic Parts and Composite Part

Part Name Part Number Part Function In Our Project
pck BBa_K2135000 Codes for PCK Used to overproduce intracellular ATP
petF BBa_K2135001 Codes for ferredoxin Used to overproduce reduced electron donors
TetR-pTet BBa_K2135002 Inducible promoter with addition of aTc Allowed control for genes of interest
TetR-pTet-petF BBa_K2135003 Ferredoxin production controlled by inducible promoter Allowed control on overproducing reduced electron donors
Part Name Part Number Part Function
HSP Promoter BBa_K873002 Heat activated promoter