Difference between revisions of "Team:ETH Zurich"

 
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           Design System Overview :
 
           Design System Overview :
 
           The core of our new diagnostic device consists of two and gate  
 
           The core of our new diagnostic device consists of two and gate  
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           and one irreversible switch. For more information, feel free to check our
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           and one irreversible switch. For more information, please check our
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           Design Section
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           <a href="https://2016.igem.org/Team:ETH_Zurich/Design"> Design Section</a>.
 
            
 
            
 
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             Take a look the details of our Stochastic and  
 
             Take a look the details of our Stochastic and  
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               Deterministic modular modele
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               Deterministic modular model.
 
          
 
          
 
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             Part Overview :
 
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             The core of our new diagnostic device consists of two and gte and one irreversible switch  
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             The core of our new diagnostic device consists of two and gates and one irreversible switch  
 
        
 
        
 
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        <h2>
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        A DIAGNOSTIC TOOL FOR <i>IN VIVO</i> SCREENING OF BIOMARKERS
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                        <a href="https://2016.igem.org/Team:ETH_Zurich/Description">
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                            <img src="https://static.igem.org/mediawiki/2016/6/6c/T--ETH_Zurich--Results_paw.png">
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                    </li><!--
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                        <a href="https://2016.igem.org/Team:ETH_Zurich/Parts">
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                        <a href="https://2016.igem.org/Team:ETH_Zurich/Model">
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                      <img src="https://static.igem.org/mediawiki/2016/9/9a/T--ETH_Zurich--wikihome.png">
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                 <h2>THE PROJECT IN DETAILS</h2>
 
                 <h2>THE PROJECT IN DETAILS</h2>
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                 <h2>CONTACT</h2>
 
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                     <li>Email: <a href="mailto:igem2016@hiddendomain" onclick="this.href=this.href.replace(/hiddendomain/,'bsse.ethz.ch')">igem2016</a>
 
                     <li>Email: <a href="mailto:igem2016@hiddendomain" onclick="this.href=this.href.replace(/hiddendomain/,'bsse.ethz.ch')">igem2016</a>
 
                     <li>Facebook: <a href="https://www.facebook.com/iGEM.ETH.Zurich/">/iGEM.ETH.Zurich</a>
 
                     <li>Facebook: <a href="https://www.facebook.com/iGEM.ETH.Zurich/">/iGEM.ETH.Zurich</a>
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                 <h2>REFERENCES</h2>
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                 <h2>REFERENCES</h2>s
 
                 <ul>
 
                 <ul>
 
                     <li><a name="cit1" class></a>[1] Maciej Chichlowski and Laura P Hale. “Bacterial-mucosal interactions in inflammatory bowel disease: an alliance gone bad”. In: <i>American Journal of Physiology-Gastrointestinal and Liver Physiology</i> 295.6 (2008), G1139–G1149.
 
                     <li><a name="cit1" class></a>[1] Maciej Chichlowski and Laura P Hale. “Bacterial-mucosal interactions in inflammatory bowel disease: an alliance gone bad”. In: <i>American Journal of Physiology-Gastrointestinal and Liver Physiology</i> 295.6 (2008), G1139–G1149.
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Latest revision as of 01:02, 3 December 2016

PAVLOV'S COLI

A DIAGNOSTIC TOOL FOR IN VIVO SCREENING OF BIOMARKERS

ABSTRACT

Inflammatory bowel disease (IBD) results in chronic inflammation of the intestines. Current diagnostic methods are invasive and rely on biomarkers that are not sufficiently disease-specific. We have engineered E. coli to detect several disease-specific biomarkers, memorize this event, and allow specific readout of the memory state. While the sensor cells travel through the gut, simultaneously occurring signals are memorized by activating an AND gate which triggers a recombination-based unidirectional switch and commits the observation to memory. After isolation from the patient’s faeces, the memory can be read out through the expression of a fluorescent protein induced by the addition of the candidate biomarker. Thus a single fluorescent protein can differentiate between many different candidate markers. A community of sensor cells can be utilized at the same time, enabling a high degree of multiplexing. Pavlov’s Coli is a non-invasive diagnostic tool for a large selection of specific biomarkers associated with IBD.

CONTACT

Thanks to the sponsors that supported our project: