Team:ETH Zurich/Composite Part

PAVLOV'S COLI

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COMPOSITE PARTS

Part Name	Biobrick	Comments
AHL regulated sfgfp expression	BBa_K2116070	BBa_J23118 promoter with a downstream esabox, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHLregulated sfgfp expression	BBa_K2116052	BBa_J23118 promoter with two downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHL regulated sfgfp expression	BBa_K2116071	BBa_J23118 promoter with three downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHL regulated sfgfp expression	BBa_K2116072	BBa_J23118 promoter with two downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
AHL regulated sfgfp expression	BBa_K2116073	BBa_J23118 promoter with three downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116074	Nitric oxide responsive PnorV promoter with its native spacer and one downstream esabox, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116075	Nitric oxide responsive PnorV promoter with its native spacer and two downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116076	Nitric oxide responsive PnorV promoter with its native spacer and three downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116077	Nitric oxide responsive PnorV promoter without its native spacer and one downstream esabox, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116078	Nitric oxide responsive PnorV promoter without its native spacer and two downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116079	Nitric oxide responsive PnorV promoter without its native spacer and three downstream esaboxes 8bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116080	Nitric oxide responsive PnorV promoter with its native spacer and two downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116081	Nitric oxide responsive PnorV promoter with its native spacer and three downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116082	Nitric oxide responsive PnorV promoter without its native spacer and two downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116083	Nitric oxide responsive PnorV promoter without its native spacer and three downstream esaboxes 15bp apart, in front of sfgfp. Expression induced in presence of both nitric oxide and the AHL 3OC6HSL.
bxb1 integrase under Ptet promoter	BBa_K2116056	bxb1 integrase with a very fast ssrA degradation tag, under the control of Ptet.
tp901 integrase under Ptet promoter	BBa_K2116057	tp901 integrase with a very fast ssrA degradation tag under the control of Ptet.
superfolder gfp under Ptet promoter	BBa_K2116085	sfgfp under the control of Ptet.
mNectarine under Ptet promoter	BBa_K2116086	mNectarine under the control of Ptet.
Nitric Oxide and AHL regulated sfgfp expression	BBa_K2116041	Nitric oxide responsive PnorV promoter with its native spacer, one esabox before the norR binding site and one downstream of transcription start site. This and gate expresses sfgfp in presence of both nitric oxide and the AHL 3OC6HSL.
mNectarine under Ptet promoter	BBa_K2116065	mNectarine with a moderately fast ssrA degradation tag, under the control of Ptet.
Dual reporter for integrase tp901	BBa_K2116058	Constitutive promoter J23118 flanked by attP and attB sites of tp901 integrase, initially expressing mNectarine. Once flipped, the reporter expresses sfgfp.
Dual reporter for integrase bxb1	BBa_K2116024	Constitutive promoter J23118 flanked by attP and attB sites of bxb1 integrase, initially expressing mNectarine with moderately fast ssrA degradation tag. Once flipped, the reporter expresses sfgfp.
bxb1 integrase under Ptet promoter	BBa_K2116061	bxb1 integrase with a moderately fast ssrA degradation tag, under the control of Ptet.
tp901 integrase under Ptet promoter	BBa_K2116062	tp901 integrase with a moderately fast degradation tag under the control of Ptet.
bxb1 integrase under Ptet promoter	BBa_K2116063	bxb1 integrase under the control of Ptet.
tp901 integrase under Ptet promoter	BBa_K2116064	tp901 integrase under the control of Ptet.
Nitric Oxide and AHL regulated bxb1 expression	BBa_K2116059	Nitric oxide responsive PnorV promoter with its native spacer, one esabox before the norR binding site and one downstream of transcription start site. This and gate expresses bxb1 with a moderately fast degradation tag, in presence of both nitric oxide and the AHL 3OC6HSL.
Nitric Oxide and AHL regulated bxb1 expression	BBa_K2116028	Nitric oxide responsive PnorV promoter with its native spacer, one esabox before the norR binding site and one downstream of transcription start site. This and gate expresses bxb1 in presence of both nitric oxide and the AHL 3OC6HSL.
Constitutively expressed transcriptional repressor EsaR	BBa_K2116087	EsaR expressed under the control of constitutive promoter J23118. EsaR is responsive to the AHL 3OC6HSL.
Nitric oxide regulated sfgfp	BBa_K2116088	Nitric Oxide responsive promoter controlling sfgfp expression.

Thanks to the sponsors that supported our project:

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