Team:ETH Zurich/Basic Part

PAVLOV'S COLI

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BASIC PARTS

Part Name	Biobrick	Comments
Transcriptional regulator esaR	BBa_K2116001	Transcriptional repressor, binds esaboxes.
mNectarine	BBa_K2116016	Red fluorescent protein mNectarine.
mNectarine+ssrA tag	BBa_K2116018	Red fluorescent protein mNectarine with a moderately fast ssrA degradation tag
bxb1 integrase+ssrA	BBa_K2116009	bxb1 integrase with a fast ssrA degradation tag
tp901 integrase+ssrA	BBa_K2116010	Red fluorescent protein mNectarine with a fast ssrA tag
pNorV	BBa_K2116002	Nitric Oxide responsive promoter
superfolder GFP	BBa_K2116017	Fast maturing and stable green fluorescent protein
NO and AHL responsive AND gate	BBa_K2116011	This is an AND gate that is repressed by EsaR and can only be activated by NorR. It requires presence of 3OC6HSL and nitric oxide for transcription.
bxb1 integrase	BBa_K2116026	bxb1 integrase, can flip any sequence flanked by its attP and attB binding sites.
tp901 integrase	BBa_K2116035	tp901 integrase, can flip any sequence flanked by its attP and attB binding sites.
EsaR regulated promoter	BBa_K2116025	The Bba_J23118 promoter with an additional esabox downstream of transcription start site. Transcription initiation only in presence of 3OC6HSL.
EsaR regulated promoter	BBa_K2116066	The Bba_J23118 promoter with two esaboxes downstream of transcription start site, separated by an 8bp spacer. Transcription initiation only in presence of 3OC6HSL.
EsaR regulated promoter	BBa_K2116037	The Bba_J23118 promoter with two esaboxes15bp spacer. Transcription initiation only in presence of 3OC6HSL.
Lactate regulated AND gate	BBa_K2116027	pNorV promoter flanked with LldR binding sites. Binding site 1 is located upstream of the pNorV promoter and binding site 2 is located after the sigma 45 binding site and before the transcription start site. The promoter will be active when both NO and lactate are present at the same time.
Lactate regulated promoter	BBa_K2116049	Promoter with LldR binding sites on each side and a spacer and J23118 promoter in between. This promoter is intended to mimic BBa_K2116027 without the NO binding function.
NO and AHL regulated AND gate	BBa_K2116004	PnorV promoter with its native spacer after transcription start site, followed by one esabox. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL.
NO and AHL regulated AND gate	BBa_K2116005	PnorV promoter with its native spacer after transcription start site, followed by two esaboxes 8bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL.
NO and AHL regulated AND gate	BBa_K2116006	PnorV promoter with its native spacer after transcription start site, followed by three esaboxes 8bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL.
NO and AHL regulated AND gate	BBa_K2116012	PnorV promoter without its native spacer after transcription start site, followed by one esabox. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL.
NO and AHL regulated AND gate	BBa_K2116013	PnorV promoter without its native spacer after transcription start site, followed by two esaboxes 8bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL.
NO and AHL regulated AND gate	BBa_K2116067	PnorV promoter without its native spacer after transcription start site, followed by three esaboxes 8bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL.
NO and AHL regulated AND gate	BBa_K2116007	PnorV promoter with its native spacer after transcription start site, followed by two esaboxes 15bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL.
NO and AHL regulated AND gate	BBa_K2116008	PnorV promoter with its native spacer after transcription start site, followed by three esaboxes 15bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL.
NO and AHL regulated AND gate	BBa_K2116068	PnorV promoter without its native spacer after transcription start site, followed by two esaboxes 15bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL.
NO and AHL regulated AND gate	BBa_K2116069	PnorV promoter without its native spacer after transcription start site, followed by three esaboxes 15bp apart. This AND gate is active in presence of nitric oxide and the AHL 3OC6HSL.

Thanks to the sponsors that supported our project:

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