Team:Stony Brook/Parts

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New BioBricks

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Cripto-1 Expression System (K1908001)

This part is a semi-composite part that was synthesized by IDT which holds a Saccharomyces cerevisiae Kozak sequence, a coding sequence for the Homo sapien membrane protein Cripto-1, and an ADH1 Terminator region. The coding sequence was codon-optimized for expression in yeast using the proprietary codon optimization program on the Integrated DNA Technologies website. Our design was intended to induce a strong expression of Cripto-1 in yeast to test for membrane localization. We hope that our contribution of a eukaryotic receptor protein to the iGEM Registry is found to be useful and beneficial.

Hepatitis B Antigen Expression System (K1908002)

BioBrick Improvements

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fwYellow-amilCP Osmolarity Color Toggle (K1908000)

This system is intended to be a blue-yellow color toggle that expresses fwYellow (K1033910) and amilCP (K592009) dependent on the activation of the OmpC promoter at varied ambient osmolarities. A negative feedback loop inhibits production of fwYellow (regulated by pLac) when the OmpC promoter is active and amilCP/LacI is being produced. Growth in high-osmolarity media is expected to give a blue color while growth in low-osmolarity media is expected to produce a yellow color. Further characterization of the construct is necessary to understand the mathematical relationship between protein expression levels at varied ambient osmolarities.

Parts

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Part Name	Part Description
BBa_K1908000	BY-Toggle
BBa_K1908001	Cripto-1 Coding Sequence
BBa_K1908002	TBA