Team:Denver Biolabs/Proof
Team:Denver_Biolabs
Proof of Concept
Our goal was to create a system to allow yeast cultures to be dried to facilitate storage and transportation.
To accomplish this we built a scaled down model of an industrial yeast drying system. To test our system we
used store-bought dried bakers yeast (saccharomyces cerevisiae). We chose to use store-bought yeast because we
did not want to risk releasing any laboratory strains of yeast while testing our system. For our test we used
Kroger brand ‘Active Dry Yeast’ packets. We followed the manufacturers instructions to reconstitute the yeast:
for each 7g packet of dry yeast we added 60ml of warm distilled water and 4g of sucrose (table sugar).
We allowed the mixture to reconstitute for 10 minutes before using in our system.
To test if our system was able to dry yeast in a viable form, we compared cells from the freshly reconstituted
active yeast packets with cells that had reconstituted from the powder produced by our spray drier. To do this
we plated 200ul of freshly reconstituted yeast on YPD native plates, spread for single colonies, and allowed
the cells to grow at 30° celsius for 24 hours. To get single colonies from yeast that had been dried with our
spray dryer, we sprinkled the powdered product directly onto YPD native plates and grew them at 30° celsius for 24 hours.
We compared the morphology of the cells with an optical microscope using methylene blue dye. To obtain our slides we mixed
a single colony with 10ul of methylene blue dye in a 1.5ml microcentrifuge tube, placed 5ul of the mixture onto a glass slide,
and placed a cover slip over the sample. We found identical morphologies between yeast cells that had been reconstituted
and dried with our system and cells that had just been reconstituted from the packet.
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