Team:NTU-Singapore/Attributions

Thanks for the help and guidance!

We are very gratefull that we have these super helpful labmates that are willing to spend time teaching us! They are the key to our success! :)

Advisors

Asst. Prof Tan Meng How

Assembled the team and met with all the team members every week to provide guidance and set research directions. Designed gRNAs for project Evaluation and primers for genomic PCR.

Dr. Teh Min Yan

Mentored Fanxiang and Huanwen for Project Evolution. Taught us mammalian tissue culture

Dr. Yuan Ming

In-charge of Project Evaluation

Liu Kaiwen Ivy

Mentored us on Project Evaluation, taught us experiments involved in T7E assay.

Adventurers

Ooi Kean Hean

Student leader of the team. Designed the wiki, Cas9 models and exhibition items. In charge on Project Truncation, analysed crystal structure of Sp-dCas9 for determination of truncations, Gibson cloning of truncation regions, maintanence of HEK293FT cells and preparation for transfection, did flow cytometry and analysis of the results for Project Truncation.

Yong Jing Yen

Responsible for submission of parts and experiments involved in collaboration. Designed Cas9 models, poster and exhibition items. Cloned constructs for Project Evaluation and carried out T7E assay, transfection of HEK293 cells, FACS preparation and genomic PCR

Liu Zhehui

Designed the brochure for our exhibition. Designed the wiki. Photographer of our team. Helped out in Project Truncation, i.e. Gibson cloning of truncation regions, preparation of HEK293FT cells for transfection, flow cytometry, and derived truncation regions in the PI domain. Cloned constructs for Project Evaluation and carried out T7E assay and genomic PCR

Li Fanxiang

Carried out all the assays involved in Project Evolution. Photographer of our team. Drew all the fantastic picture of us!

Mu Huanwen

Carried out all the assays involved in Project Evolution and testing the mutant Cas9 in HEK 293 cells

Zhang Junyi

Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, and Surveyor Assay.

Shaun Lim Wen Zheng

Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, and Surveyor Assay

NORFALA-ALIAH BINTE SUTRISNOH

Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, Surveyor Assay

YEO HWEE TING

Carried out experiments of Project Evalutiaon like T7E assay, transfection of HEK293 cells, FACS preparation, genomic PCR, Surveyor Assay

Wong Ming Jie

Helped out during our exhibition

Bui Thuy Thien

Helped out during our exhibition

Jenndy

Helped out during our exhibition

3D printing support

Dr. Bai Jiaming, A*STAR SIMTECH

Dr. Bai was kind enought to help us print out our 3D models with the MakerBotX

FACS and Flow cytometry support

Dr. Abdul Rashid Bin Mohammad Muzaki

Rashid is the Flow-facility Assistant Manager who was very kind to teach us how to operate the FACSDiva software and the Fortessa X20 flow cytometry machine.

Last but not least, our sponsors!