General

Logistics

Yifan Chen, Yi Liu

Abstract

Yi Liu, Fang Ba, Shijie Gu

Parts

Shijie Gu, Yifan Chen

Webpage

Yifei Shen

wiki Big Picture

Lechen Qian, Fang Ba

wiki Design

Yifei Shen

wiki Home Background

Qinying Ye

wiki Home Video*

Shijie Gu, Jiaming Zhang, Qinying Ye, Lechen Qian

wiki Logo

Qinying Ye

wiki Team Avatar

JSNU

Banner&Post

Fang Ba

Special Notes:iGEM is the first lab experience for many members on our team. It was the training and guidance of the host labs that put us onto the right track for carrying out the independent project, Solar Hunter. On this page, we show our acknowledgement sincerely by clearly attributing the help from the graduate students and instructors at the beginning of each section, as different graduate students were involved in different aspects of the project.
*Wiki Video Citation Information: Tone of Clostridium. acetobutylicum: https://www.youtube.com/watch?v=wNzrhCq_2gU; Background Music: NERDHEAD - Life is Beautiful (Instrumental),(https://www.youtube.com/watch?v=gQkBr1I19Ys).

InterLab

Yi Liu, Fang Ba, Zhu Haolong, Qiaoqi Wang

Special Notes:We applied for the flow cytometry through the manager teacher Ren Xiaoyue. She also tutored us the basic operation and the preparation of our samples. We prepared the samples by ourselves.

Hydrogenase Section

Clone Design

Yilin Sun, Yifan Chen, Yan Nie, Yi Liu

Sequencing Confirm

Yi Liu, Yifan Chen

Clone hydrogenase gene clusters

Yi Liu, Fang Ba, Yifan Chen, Haonan Yang

Cre recombination&Result Demonstration

Fang Ba, Yi Liu

Hydrogen Assay

Yi Liu, Fang Ba, Qixi Mi, Zetao Ding

Preparation of component cell

Yi Liu, Yifan Chen

HydA Induction, Purification and WB Demonstration

Haolong Zhu, Qiaoqi Wang

Wiki of Hydrogenase

Yi Liu, Shijie Gu, Fang Ba, Lei Zhuang

Special Notes:In this part Yan Nie provided us with the original bacteria of PirLC to make the component cell and the transformed PirLC with the plasmid backbone.The molecular cloning in this section was done in Yan Nie’s Lab, SIAIS. The help of Yan Nie was involved in the the beginning, but the principle is that all of the experiments are done by ourselves. Yilin Sun provided us with the tutorials of Snapgene and primer design.The sequencing is provided by Tsingke Sequencing and Jie Li Sequencing.Qixi Mi is a PI from School of Physical Science and Technology. Zetao Ding is his Ph.D student. In the hydrogen production assay, they provided us with the instrument/apparatus he used for hydrogen production assay in his lab. He trained for using the apparatus and most of the experiments were conducted by us.

Biofilm Section

Clone of a library of CsgA fused protein and sequencing confirm

Shijie Gu, Bolin An (Graduate Instructor/Advisor)

Clone of CsgA-his

A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center

Clone of mcherry-SpyTag

Shijie Gu

mCherry-SpyCatcher strain

A gift of Chao Zhong Lab at ShanghaiTech University

CsgA-KO stra in

A gift of Timothy K. Lu Lab at MIT Synthetic Biology Center

Colony PCR

Shijie Gu, Lechen Qian

Biofilm Characterization

Lechen Qian, Jiaming Zhang

Biofilm culture

Lechen Qian, Lei Zhuang, Weitong Zheng

Different nanomaterials supply for characterization

Xinyu Wang, Jiahua Pu (Graduate Instructor/Advisor), Jiaming Zhang, Lei Zhuang

Biofilm expression optimization

Lechen Qian

biofilm data analysis and result demostration

Lechen Qian

notebook data and protocal collection

Weitong Zheng

wiki of biofilm

Lechen Qian, Shijie Gu

wiki biofilm picture

Lechen Qian

Special Notes: Bolin An is the leading graduate instructor/advisor in this section. He provided basic cloning training (PCR, restriction, ligation, Gibson, electrophoresis, Colony PCR, blast) at the beginning of the cloning of his-CsgA-SpyCatcher-his, the purification of proteins at the subsection of Expression and Purification of mCherry-SpyTag, and consult throughout the summer and fall. In addition, all florescent microscope experiments were conducted with the supervision of Bolin An. TEM is conducted with the help of Xinyu Wang. However, the preparation of the samples were done by ourselves.

Nano material Section

Quantum dots, nanorods and ligand synthesis

Jiaming Zhang, Lei Zhuang, Kang Wang

Quantum dot and nanorods characterization

Jiaming Zhang

wiki of Quantum Dots

Jiaming Zhang, Lei Zhuang

Special Notes: Jiahua Ou and Xinyu Wang are two graduate instructors/advisors in the QD section. Pu provided guidance on our experiments including the synthesis of CdSe QDs and CdS NRs and UV-Vis spectrometer characterization. Wang helped in the synthesis of NTA ligand, TEM characterization and PL spectrometer characterization.

Human Practice Section

Team Collaborations

Yi Liu, Haolong Zhu, Lechen Qian, Fang Ba

Public Questionnaire

Haolong Zhu, Yi Li, Lechen Qian, Qiaoqi Wang

Social Interviews

Haolong Zhu. Yi Liu, Jiaming Zhang, Lechen Qian, Yifan Chen

Propagation of iGEM

Haolong Zhu; Shijie Gu, Lechen Qian; Weitong Zheng

Wiki of Human Practice

Haolong Zhu, Fang Ba

Team:ShanghaitechChina/Attributions

General

InterLab

Special Notes:We applied for the flow cytometry through the manager teacher Ren Xiaoyue. She also tutored us the basic operation and the preparation of our samples. We prepared the samples by ourselves.

Hydrogenase Section

Biofilm Section

Nano material Section

Human Practice Section