Week 2 (7/5-7/8)
7/5
- Ran the PCR'ed construct on a gel with Phire Polymerase → It didn't work
7/6
- Ran the construct with PCR on gel → it worked
- Tasnia stabbed our gel
- Interlab study continues
7/7
- Nanodrops were pretty low (84-120 ng/nl)
- 11:46am PCR'ed the highest nanodrop result in tube #1 → PCR successful
- 2:33pm → attempt to digest with remaining non-PCR product → removed from incubator at 5:00pm
- Made a gel → ran PCR