Team:Stony Brook/Notebook/Vaccine-W3

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Week 3: 7/11-7/15

Week 3 (7/11-7//15)

7/11

Vaccine Group
  • Stuff & Things

7/12

Vaccine Group
  • Stuff & Things

7/13

Vaccine Group
  • Stuff & Things

7/14

Vaccine Group
    Contents of well Amount (ul)
    Ladder 10
    AD 5
    AO 5
    AM 5
    AN 5
    Gal-Sunil Added 1ul loading dye
    Con-Sunil 5
  • Brian and Sunil's Gal promoters worked but both constructs failed

7/15

Vaccine group
  • Jon and Sunil retrying construct
  • Jon used phire at 50°C
  • Sunil used Q5. Extension at 72°C for 30 seconds
    Polymerase Primers (ul) Template (ul) H2O (ul)
    Phire (25ul) 2.5 Reverse Construct 2.5 Forward Stitch 1.5 19.5
    Q5 (25ul) 2.5 Reverse Construct 2.5 Forward Stitch 1.5 19.5