Team:Stony Brook/Notebook/Vaccine-W5

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Week 4: 7/25-

7/25

Ran a gel

Lanes:

  1. Ladder
  2. GCL 24
  3. GC
  4. GCL 21
  5. Con 25
  6. Gal 25
  7. BCP 25
Nanodrop of PCR Purification
    Content Concentration (ng/ul)
    BCP 7/25 39.9
    Con 7/25 130.1
    Gal 7/25 130.7
    Digestions

    Construct (130.1ng/ul)
      Content ul
      H2O 27.6
      Buffer 5
      EcoRI 1
      SpecI 1
      DNA 15.4

    PDN-G1GZ (17.1 ng/ul)
      Content ul
      H2O 5.5
      Buffer 5
      EcoRI 0.5
      SpecI 0.5
      DNA 2.5

    PSB-1CB (315.9 ng/ul)
      Content ul
      H2O 36.7
      Buffer 5
      EcoRI 1
      SpecI 1
      DNA 6.33

7/26

Ran a gel on 7/25's digests

Lanes:

  1. Ladder
  2. Con 7/26
  3. PDN-G1GZ
  4. PSB-1CB
  • Heat inactivated remaining volume of digestions in heat bath
  • Heat bath: 20 minutes at 80°C
Four PCR reactions of Construct using Q5
    Content Amount (ul)
    Q5 12.5
    BB Prefix .125
    BB Suffix 1.25
    Construct 0.5
    Nuclease-free water 9.5
Ran a gel on the PCR Products

Lanes

  1. Ladder
  2. Construct Digest
  3. PPN Digest
  4. PSB Digest
  5. Construct Q5 #1
  6. Construct Q5 #2
  7. Construct Q5 #3
  8. Construct Q5 #4