Difference between revisions of "Team:NCTU Formosa/Composite Part"

Line 77: Line 77:
 
}
 
}
  
−
/*scroll navigaiton*/
 
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nav {
 
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  padding: 18px;
 
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  position: fixed;
 
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  height: auto;
 
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  left:0.5vw;
 
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  width: auto;
 
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  z-index: 9999;
 
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  top:25%;
 
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  margin: auto;
 
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}
 
−
 
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nav .wrapper {
 
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  padding: 0;
 
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  width:100%;
 
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  height: 785px;
 
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}
 
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nav .nodes {
 
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  position: relative;
 
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  margin-top: 1px;
 
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  margin-left: 1px;
 
−
 
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}
 
−
 
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.line {
 
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  position: absolute;
 
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  width:1px;
 
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  height:705px;
 
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  top: 13px;
 
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}
 
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.line .red {
 
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  width:1px;    /*調line長度*/
 
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  height:50%; /*line寬度*/
 
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  position:relative;
 
−
 
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}
 
−
 
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.line .blue {
 
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  width: 1px;  /*line寬度*/
 
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  height: 20%;  /*調line長度*/
 
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  position:relative;
 
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  left:51px;
 
−
 
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}
 
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.line .red-container {
 
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  position:relative;
 
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  width: 4px;
 
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}
 
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.line .red {
 
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  background-color:#00D6FF;/*cover line color*/
 
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  *zoom: 1;
 
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  background-size: 100%;
 
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}
 
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.line .blue {
 
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  background-color: white;/*line color*/
 
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  *zoom: 1;
 
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  background-size: 100%;
 
−
 
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}
 
−
 
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.node {
 
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  margin-left: 40px;/*調node間距*/
 
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  width: 20px;
 
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  height: 20px;
 
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  margin-top: 30px;
 
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  position: relative;
 
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}
 
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.node .splash {
 
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  box-shadow: 0px 0px 8px 2px rgba(69, 174, 233, 0.53);
 
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  transform: translateZ(0px) scale(1);
 
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  position: absolute;
 
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  width: 20px;
 
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  height: 20px;
 
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  border-radius: 100%;
 
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  pointer-events: none;
 
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  opacity: 1;
 
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}
 
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.node.active .active-circle {
 
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  transform: scale(1);
 
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  opacity: 1;
 
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  background:white;/*active circle color*/
 
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  left:1.6px; 
 
−
}
 
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.node.active .splash {
 
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  transition: transform 1200ms, opacity 1500ms;
 
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  transform: translateZ(0px) scale(2.5);
 
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  opacity: 0 !important;
 
−
 
−
}
 
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.node.active .inactive-circle {
 
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  transition: opacity 200ms;
 
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  opacity: 0;
 
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}
 
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.node .active-circle {
 
−
  transition: all 300ms cubic-bezier(0.63, 0.62, 0.48, 1.84);
 
−
  transform: scale(0.7);
 
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  position: absolute;
 
−
  opacity: 0;
 
−
  width: 20px;
 
−
  height: 20px;
 
−
  border-radius: 100%;
 
−
  background-color: #4b1202;
 
−
  *zoom: 1;
 
−
  background-size: 100%;
 
−
 
−
}
 
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.node .active-circle .cover {
 
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  transform: translateZ(0px);
 
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  width: 16px;
 
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  height: 16px;
 
−
  background:#00D6FF;/*active circle color */
 
−
  border-radius: 100%;
 
−
  position: absolute;
 
−
  top: 2px;
 
−
  left: 2px;
 
−
}
 
−
 
−
.node .inactive-circle {
 
−
  transition: all 300ms cubic-bezier(0.63, 0.62, 0.48, 1.84);
 
−
  width: 14px;
 
−
  height: 14px;
 
−
  display: block;
 
−
  background: #000;
 
−
  position: absolute;
 
−
  border-radius: 100%;
 
−
  border: 2px solid white;
 
−
  top: 3px;
 
−
  left: 3px;
 
−
}
 
−
.node:hover {
 
−
  cursor: pointer;
 
−
}
 
−
.node:hover .active-circle {
 
−
  transition: all 200ms cubic-bezier(0.63, 0.62, 0.48, 1.84);
 
−
  transform: scale(0.84);
 
−
}
 
−
 
−
.node:hover .inactive-circle {
 
−
  transition: all 200ms cubic-bezier(0.63, 0.62, 0.48, 1.84);
 
−
  transform: scale(0.85);
 
−
}
 
  
 
/*left-topic*/
 
/*left-topic*/
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     margin-top:20px;
 
     margin-top:20px;
 
}
 
}
−
.title-{
+
.title-1{
 
     font-size:25pt !important;
 
     font-size:25pt !important;
 
     text-shadow:1px 1px 10px rgb(0, 206, 255);
 
     text-shadow:1px 1px 10px rgb(0, 206, 255);

Revision as of 14:02, 12 October 2016

Composite part

We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria E. coli BL21 Rosetta Gami strain. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of E. coli we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.

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