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<p class="title">Composite part</p> | <p class="title">Composite part</p> | ||
− | <p class="content">We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide(OAIP) respectively and they are easy to express with the help of the bacteria <i>E. coli BL21 Rosetta Gami strain</i>. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of <i>E. coli</i> we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.</p> | + | <p class="content">We have known the function of the three toxic peptides of Pantide, ω-hexatoxin-Hv1a, µ-segestritoxin-Sf1a, and Orally Active Insecticidal Peptide (OAIP) respectively and they are easy to express with the help of the bacteria <i>E. coli BL21 Rosetta Gami strain</i>. So, we then start to create BioBricks of these three toxins which we have obtained the nucleotide sequences. We use a strong T7 promoter that is suitable with the strain of <i>E. coli</i> we chose and also powerful at expressing proteins with multiple disulfide bonds, and we add His-tag for protein purification.</p> |
<p class="title">Pantide without lectin</p> | <p class="title">Pantide without lectin</p> |
Revision as of 14:32, 12 October 2016