Difference between revisions of "Team:SUSTech Shenzhen/Project"

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= Welcome to iGEM 2016! =
 
= Welcome to iGEM 2016! =

Revision as of 16:12, 12 October 2016

Team SUSTC-Shenzhen

Project Overview

test page

Abstract

Audiogenetics is a useful tool for high-efficiency cell regulation. Compared to chemical genetics, it stimulates cells with better precision and less toxicity. Furthermore, signals are conveyed to target cells with little delay, leading to a shorter response time. To achieve our goal of audiogenetics, membrane mechanosensitive channels (TRPC5 and Piezo) are chosen as receptors. Fluorescent calcium indicator (R-GECO) is employed to indicate cytoplasmic calcium level. Nuclear factor of activated T cells (NFAT) and YFP are used as downstream indicators to quantify the regulatory abilities.

SUSTech Shenzhen-ElementisLogo.jpg
Elementis Logo

Microfluidic channels are also utilized in the pre-study in which shear stress, similar to sound waves, is applied on cell surface as signal input to explore the basic parameters. A sound generator is constructed to test whether sound can trigger the channels as expected. Additionally, we use directed evolution to improve channels' selectivity for specific sound frequency and their sensitivity to sound of lower intensity.


fff a
DNA (500ng) 8ul
Cutsmart buffer 2.5ul
EcoRI-HF 0.5ul
XbaI 0.5ul
ddH2O 16.5ul
Total 25ul
msfGFP CDS① msfGFP CDS②
DNA(300ng) 2.5ul 2.5ul
Cutsmart buffer 1ul 1ul
MluI-HF/MscI 0.5ul 0.5ul
ddH2O 6ul 6ul
Total 10ul 10ul

Welcome to iGEM 2016!

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Your team has been approved and you are ready to start the iGEM season!

Before you start

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Please read the following pages:

Styling your wiki

You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.

SUSTech Shenzhen-20160907,osmotic.png
test

While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.


Wiki template information

We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!

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On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

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Tips

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

  • State your accomplishments! Tell people what you have achieved from the start.
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  • Avoid using very small fonts and low contrast colors; information should be easy to read.
  • Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a>
  • Have lots of fun!



Inspiration

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Made by from the iGEM team SUSTech_Shenzhen.

Licensed under CC BY 4.0.

  • ©2016 SUSTech_Shenzhen