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Another technology used is directed evolution. Here, we apply random PCR to acquire a large library of mutants of mechanosensitive channels which are then screened and selected based on the downstream pathways we design to meet our needs. --> | Another technology used is directed evolution. Here, we apply random PCR to acquire a large library of mutants of mechanosensitive channels which are then screened and selected based on the downstream pathways we design to meet our needs. --> | ||
− | Audiogenetics is a useful tool for high-efficiency cell regulation. Compared to chemical genetics, it stimulates cells with better precision and less toxicity. Furthermore, signals are conveyed to target cells with little delay, leading to a shorter response time. | + | Audiogenetics is a useful tool for high-efficiency cell regulation. Compared to chemical genetics, it stimulates cells with better precision and less toxicity. Furthermore, signals are conveyed to target cells with little delay, leading to a shorter response time.{{SUSTech_Shenzhen/math|equ=<nowiki>f(x) = \int_{-\infty}^\infty |
− | + | ||
− | {{SUSTech_Shenzhen/math|equ=<nowiki>f(x) = \int_{-\infty}^\infty | + | |
\hat f(\xi)\,e^{2 \pi i \xi x} | \hat f(\xi)\,e^{2 \pi i \xi x} | ||
\,d\xi</nowiki>}} | \,d\xi</nowiki>}} | ||
To achieve our goal of audiogenetics, membrane mechanosensitive channels (TRPC5 and Piezo) are chosen as receptors. Fluorescent calcium indicator (R-GECO) is employed to indicate cytoplasmic calcium level. Nuclear factor of activated T cells (NFAT) and YFP are used as downstream indicators to quantify the regulatory abilities.{{SUSTech_Image | filename=SUSTech_Shenzhen-ElementisLogo.jpg | caption=Elementis Logo}} | To achieve our goal of audiogenetics, membrane mechanosensitive channels (TRPC5 and Piezo) are chosen as receptors. Fluorescent calcium indicator (R-GECO) is employed to indicate cytoplasmic calcium level. Nuclear factor of activated T cells (NFAT) and YFP are used as downstream indicators to quantify the regulatory abilities.{{SUSTech_Image | filename=SUSTech_Shenzhen-ElementisLogo.jpg | caption=Elementis Logo}} | ||
+ | |||
+ | {{SUSTech_Shenzhen/bmath|equ=<nowiki>f(x) = \int_{-\infty}^\infty | ||
+ | \hat f(\xi)\,e^{2 \pi i \xi x} | ||
+ | \,d\xi</nowiki>}} | ||
Microfluidic channels are also utilized in the pre-study in which shear stress, similar to sound waves, is applied on cell surface as signal input to explore the basic parameters. A sound generator is constructed to test whether sound can trigger the channels as expected. Additionally, we use directed evolution to improve channels' selectivity for specific sound frequency and their sensitivity to sound of lower intensity. | Microfluidic channels are also utilized in the pre-study in which shear stress, similar to sound waves, is applied on cell surface as signal input to explore the basic parameters. A sound generator is constructed to test whether sound can trigger the channels as expected. Additionally, we use directed evolution to improve channels' selectivity for specific sound frequency and their sensitivity to sound of lower intensity. |
Revision as of 16:18, 14 October 2016
![](https://2016.igem.org/wiki/images/6/61/T--SUSTech_Shenzhen--LOGO_nowords.png)
Project Overview
test page
Contents
Abstract
Audiogenetics is a useful tool for high-efficiency cell regulation. Compared to chemical genetics, it stimulates cells with better precision and less toxicity. Furthermore, signals are conveyed to target cells with little delay, leading to a shorter response time.f(x) = \int_{-\infty}^\infty \hat f(\xi)\,e^{2 \pi i \xi x} \,d\xi
To achieve our goal of audiogenetics, membrane mechanosensitive channels (TRPC5 and Piezo) are chosen as receptors. Fluorescent calcium indicator (R-GECO) is employed to indicate cytoplasmic calcium level. Nuclear factor of activated T cells (NFAT) and YFP are used as downstream indicators to quantify the regulatory abilities.
f(x) = \int_{-\infty}^\infty \hat f(\xi)\,e^{2 \pi i \xi x} \,d\xi
Microfluidic channels are also utilized in the pre-study in which shear stress, similar to sound waves, is applied on cell surface as signal input to explore the basic parameters. A sound generator is constructed to test whether sound can trigger the channels as expected. Additionally, we use directed evolution to improve channels' selectivity for specific sound frequency and their sensitivity to sound of lower intensity.
a | |
---|---|
DNA (500ng) | 8ul |
Cutsmart buffer | 2.5ul |
EcoRI-HF | 0.5ul |
XbaI | 0.5ul |
ddH2O | 16.5ul |
Total | 25ul |
msfGFP CDS① | msfGFP CDS② | |
---|---|---|
DNA(300ng) | 2.5ul | 2.5ul |
Cutsmart buffer | 1ul | 1ul |
MluI-HF/MscI | 0.5ul | 0.5ul |
ddH2O | 6ul | 6ul |
Total | 10ul | 10ul |
a | |
---|---|
DNA (500ng) | 8ul |
Cutsmart buffer | 2.5ul |
EcoRI-HF | 0.5ul |
XbaI | 0.5ul |
ddH2O | 16.5ul |
Total | 25ul |
Welcome to iGEM 2016!
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