Revision as of 18:10, 14 October 2016

NTU-Singapore

CRISPRy to the rescue!!

The CRISPR/Cas9 system can be a beacon of hope for genetic diseases but can also be the harbinger of doom if used unethically. We investigated on the prospects of this technology by surveying the opinion of Singapore's community. On the other hand, we have combine techniques of Computer Aided Design(CAD) sofwares and PyMOL to customise proteins for 3D printing.

Protein 3D printing

CRISPR Survey

We're lucky to be in the era of 3D printing. In our opinion, science education are not only fun but cognitively stimulating when there's a model for us to play with. Hence, we decided to design 3D models of Cas9 during our exhibition. For 3D printing, all files have to be converted to .stl fromat. From the web, we have found that we can convert a .pdb file to .stl file with USCB's Chimera software.

Hence, we were very excited and we tried to print a small protein, insulin, in it's alphe helical structure as a start! However, we met few challenges.

To solve our problem, we found out that PyMOL is able to make our structures thicker and, hence, more rigid. After thickening our loop regions in PyMOL. We have to export our desired structure in .vrl format and then convert to .stl with Chimera.

Now, we can have thicker and more stable helices.

We came out with an idea to put joints into the protein to make our models more interactive and less static. We used, Autodesk Fusion to design joints for our protein. By using, Autodesk MeshMixer, we are able make modifications to our protein structure, like adding joints to our protein. More detialed instructions can be found here.

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                                  <p>Now, we can have thicker and more stable helices.</p>
-                                 <p>When we started to know how to do print a protein, we came out with an idea to put joints into the protein to make our models more interactive and less static.</p>
+                                 <p>We came out with an idea to put joints into the protein to make our models more interactive and less static. We used, Autodesk Fusion to design joints for our protein.  By using, Autodesk MeshMixer, we are able make modifications to our protein structure, like adding joints to our protein. More detialed instructions can be found here. </p>
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                                                    <img src="https://static.igem.org/mediawiki/2016/6/6a/T--NTU-Singapore--design.png" width="1200px;">

Difference between revisions of "Team:NTU-Singapore/HP/Gold"

Revision as of 18:10, 14 October 2016

CRISPRy to the rescue!!

"Protein 3D Printing" For Dummies

CRISPR Exhibition