Difference between revisions of "Team:Bordeaux"
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<div class="column full_size" > | <div class="column full_size" > | ||
| − | <img style | + | <img style="width=40%; height=40%;" src="https://static.igem.org/mediawiki/2016/a/ab/LogoTeamBordeaux.png" title="iGEM Bordeaux 2016"> |
<h1 align='center'> Sleep with EpiC <i>elegans</i> </h1> | <h1 align='center'> Sleep with EpiC <i>elegans</i> </h1> | ||
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<h3 align="justify">Background</h3> | <h3 align="justify">Background</h3> | ||
| − | <p align="justify">A 2008 report | + | <p align="justify">A 2008 report by the INPES, a French public institute, has shown that 45% of the 25-45 year-old population considers that they <b>do not sleep enough</b>. This lack of sleep causes a fair bit of <b>negative impacts</b> : decrease of focus, weakened immune system, cardiovascular disease risk increase ... Even if these statistics are high, today's medical knowledge is not sufficient enough when it comes to heal people who suffer from <b>sleep disorders</b>. A classic example would be sleeping pills for chronic insomnia. However, they only treat the symptoms, not the source. This drew our attention, enough to provoke unanimity for this project within our team.</p> |
<br><br> | <br><br> | ||
<h3 align="justify">Objectives</h3> | <h3 align="justify">Objectives</h3> | ||
| − | <p align="justify">This year, iGEM Bordeaux | + | <p align="justify">This year, iGEM Bordeaux aims to study <b>DSIP</b>, a sleep-inducing peptide which seems to be promising for the given dilemma. In order to understand in which mechanisms this peptide is involved, it will be produced by the bacteria <b><i>E. coli</i></b> and then given to the nematode <b><i>C. elegans</i></b>. It would be interesting to directly produce this peptide in <i>C. elegans</i> too.</p> |
| − | <p align="justify"> | + | <p align="justify">Another aspect, we wish to <b>control the nematode's sleep pattern</b>. On the one hand, we want to make a <b>photo-inductible system</b> that can be used on sleep genes' promoters. On the other hand, we aim to create a new tool to modify the sleep genes <b>using epigenetics</b> the sleep genes : <b>EpiCrispr</b>. Based on the CRISPR-CAS9 concept, we want to design it according to two strategies : both will use a fusion technique between a methylase and CAS9, but the second approach is inspired on Sun-Tag technique. Our final goal for this part of our project is to compare the two approach and see which one would be faster to control <i> C.elegans</i>'s sleep.</p> |
</div> | </div> | ||
Revision as of 14:07, 30 June 2016
Sleep with EpiC elegans
Who has never suffered from sleep disorders ?
Background
A 2008 report by the INPES, a French public institute, has shown that 45% of the 25-45 year-old population considers that they do not sleep enough. This lack of sleep causes a fair bit of negative impacts : decrease of focus, weakened immune system, cardiovascular disease risk increase ... Even if these statistics are high, today's medical knowledge is not sufficient enough when it comes to heal people who suffer from sleep disorders. A classic example would be sleeping pills for chronic insomnia. However, they only treat the symptoms, not the source. This drew our attention, enough to provoke unanimity for this project within our team.
Objectives
This year, iGEM Bordeaux aims to study DSIP, a sleep-inducing peptide which seems to be promising for the given dilemma. In order to understand in which mechanisms this peptide is involved, it will be produced by the bacteria E. coli and then given to the nematode C. elegans. It would be interesting to directly produce this peptide in C. elegans too.
Another aspect, we wish to control the nematode's sleep pattern. On the one hand, we want to make a photo-inductible system that can be used on sleep genes' promoters. On the other hand, we aim to create a new tool to modify the sleep genes using epigenetics the sleep genes : EpiCrispr. Based on the CRISPR-CAS9 concept, we want to design it according to two strategies : both will use a fusion technique between a methylase and CAS9, but the second approach is inspired on Sun-Tag technique. Our final goal for this part of our project is to compare the two approach and see which one would be faster to control C.elegans's sleep.
