Difference between revisions of "Team:ShanghaitechChina/InterLab"
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We participated in InterLab project this year. We completely finished the work of measuring the expressional level of GFP under different promotor through the method of flow cytometry. With following the standard protocal strictly, the data is reliable. | We participated in InterLab project this year. We completely finished the work of measuring the expressional level of GFP under different promotor through the method of flow cytometry. With following the standard protocal strictly, the data is reliable. | ||
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| + | <div id="Method" class="content"> | ||
| + | <div class="row"> | ||
| + | <div class="col-lg-12"> | ||
| + | <h1 align="center">Method</h1> | ||
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<h1 align="center">InterLab</h1> | <h1 align="center">InterLab</h1> | ||
<h3>For Negtive result</h3> | <h3>For Negtive result</h3> | ||
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/0/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/0/05/T--ShanghaitechChina--interlab--NC1.png" > |
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/c/cf/T--ShanghaitechChina--interlab--NC2.png" > |
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/1/16/T--ShanghaitechChina--interlab--NC3.png" > |
<h3>For Positive result</h3> | <h3>For Positive result</h3> | ||
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/1/1b/T--ShanghaitechChina--interlab--PC1.png" > |
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/b/b5/T--ShanghaitechChina--interlab--PC2.png" > |
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/c/cb/T--ShanghaitechChina--interlab--PC3.png" > |
<h3>For Measurement Kit I</h3> | <h3>For Measurement Kit I</h3> | ||
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/9/9c/T--ShanghaitechChina--interlab--D1-1.png" > |
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/a/ab/T--ShanghaitechChina--interlab--D1-2.png" > |
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/8/85/T--ShanghaitechChina--interlab--D1-3.png" > |
<h3>For Measurement Kit II</h3> | <h3>For Measurement Kit II</h3> | ||
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/4/4d/T--ShanghaitechChina--interlab--D2-1.png" > |
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/d/df/T--ShanghaitechChina--interlab--D2-2.png" > |
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/7/70/T--ShanghaitechChina--interlab--D2-3.png" > |
<h3>For Measurement Kit II</h3> | <h3>For Measurement Kit II</h3> | ||
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/d/d6/T--ShanghaitechChina--interlab--D3-1.png" > |
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/9/9d/T--ShanghaitechChina--interlab--D3-2.png" > |
| − | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/ | + | <img class="pic3x pic3" src="https://static.igem.org/mediawiki/2016/f/f1/T--ShanghaitechChina--interlab--D3-3.png"> |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 06:55, 17 October 2016
Abtract
InterLab is an important part in IGEM competition, whose topic is the reproducibility of protein expression(mainly GFP) from engineered biological constructs in E.coli.[1] It published an interesting but universal question， We participated in InterLab project this year. We completely finished the work of measuring the expressional level of GFP under different promotor through the method of flow cytometry. With following the standard protocal strictly, the data is reliable.Method
InterLab
For Negtive result
For Positive result
For Measurement Kit I
For Measurement Kit II
For Measurement Kit II
