Difference between revisions of "Team:Lubbock TTU"
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<div class="column full_size" > | <div class="column full_size" > | ||
| − | <h2> | + | <h2> Project Background </h2> |
| − | <p> | + | |
| + | <p>In 2010, it was estimated that 6.5 million people in the United States alone suffered from chronic wounds, accruing an annual cost of about $2.5 billion. Furthermore, experts predict that the burden of chronic wounds will increase rapidly in the near future due to increasing medical costs, an aging population, and the emergence of antibiotic resistant bacteria.</p> | ||
| + | |||
| + | <p>A chronic wound is considered a wound that does not heal in an orderly set of stages or within a time period of about three months. The etiology of chronic wounds is very diverse. One of the most prevalent reasons patients have persistent chronic wounds is that their bodies produce too many proteases at the wound site. In turn, these proteases degrade the extracellular matrix of the wound site which acts as a scaffold for new cells to migrate and grow. Proteases have also been shown to decrease healing rates by degrading growth factors that are needed for recruiting wound healing cells and inducing cellular proliferation.</p> | ||
| + | |||
| + | <p>Wound healing progresses through three successive stages known as inflammation, proliferation, and remodeling. Ultimately, the degradation of the extracellular matrix and growth factor cause the wound to remain stuck in the inflammation phase, thus unable to heal.</p> | ||
| + | |||
| + | </div> | ||
| + | |||
| + | <div class="column full_size" > | ||
| + | <h2> Project Description </h2> | ||
| + | |||
| + | <p>We will use synthetic biology principles to help treat chronic wounds by targeting the | ||
| + | overproduction of wound site protease.</p> | ||
| + | |||
| + | <UL> | ||
| + | |||
| + | <LI>For Aim 1 we will genetically engineer E. coli to produce a protease inhibitor and platelet | ||
| + | derived growth factor. | ||
| + | |||
| + | <LI>For Aim 2 we will purify the protease inhibitor and platelet derived growth factor in a | ||
| + | bioreactor. | ||
| + | |||
| + | <LI>For Aim 3 we will design a collagen bandage that mimics the human extracellular matrix, and | ||
| + | infuse it with purified protease inhibitor and platelet derived growth factor. | ||
| + | |||
| + | </UL> | ||
| + | |||
| + | <p>Our approach is two-fold. By infusing the collagen extracellular matrix with platelet derived | ||
| + | growth factor and protease inhibitor, chronic wounds should be able to progress past the | ||
| + | inflammation phase and begin healing once again.</p> | ||
</div> | </div> | ||
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<h5> Editing your wiki </h5> | <h5> Editing your wiki </h5> | ||
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p> | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p> | ||
| − | <p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> | + | <p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p> |
</div> | </div> | ||
| Line 78: | Line 108: | ||
<h5> Uploading pictures and files </h5> | <h5> Uploading pictures and files </h5> | ||
<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br /> | <p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br /> | ||
| − | When you upload, set the "Destination Filename" to <code> | + | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p> |
Revision as of 20:13, 4 July 2016
Project Background
In 2010, it was estimated that 6.5 million people in the United States alone suffered from chronic wounds, accruing an annual cost of about $2.5 billion. Furthermore, experts predict that the burden of chronic wounds will increase rapidly in the near future due to increasing medical costs, an aging population, and the emergence of antibiotic resistant bacteria.
A chronic wound is considered a wound that does not heal in an orderly set of stages or within a time period of about three months. The etiology of chronic wounds is very diverse. One of the most prevalent reasons patients have persistent chronic wounds is that their bodies produce too many proteases at the wound site. In turn, these proteases degrade the extracellular matrix of the wound site which acts as a scaffold for new cells to migrate and grow. Proteases have also been shown to decrease healing rates by degrading growth factors that are needed for recruiting wound healing cells and inducing cellular proliferation.
Wound healing progresses through three successive stages known as inflammation, proliferation, and remodeling. Ultimately, the degradation of the extracellular matrix and growth factor cause the wound to remain stuck in the inflammation phase, thus unable to heal.
Project Description
We will use synthetic biology principles to help treat chronic wounds by targeting the overproduction of wound site protease.
- For Aim 1 we will genetically engineer E. coli to produce a protease inhibitor and platelet derived growth factor.
- For Aim 2 we will purify the protease inhibitor and platelet derived growth factor in a bioreactor.
- For Aim 3 we will design a collagen bandage that mimics the human extracellular matrix, and infuse it with purified protease inhibitor and platelet derived growth factor.
Our approach is two-fold. By infusing the collagen extracellular matrix with platelet derived growth factor and protease inhibitor, chronic wounds should be able to progress past the inflammation phase and begin healing once again.
Before you start:
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2016 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples:
Uploading pictures and files
You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
