Difference between revisions of "Team:SUSTech Shenzhen/Parts"

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This year, we totally submitted 27 BioBricks, including 15 basic Parts and 12 composite Parts. We always want to contribute all of the components used in our programs and we welcome all researchers to use, measure and modify our Parts for their projects.
  
 +
We spared no effort do diversify our BioBricks and we successfully constructed BioBricks as listed below:
  
 +
{|class="table table-striped"
 +
! '''Type'''
 +
! '''Name'''
 +
! '''Description'''
 +
! '''Length'''
 +
! '''Characteristics'''
 +
|-
 +
| Basic Parts
 +
| BBa_K1943012
 +
| msfGFP CDS
 +
| 717bp
 +
| Mutated sfGFP coding sequence without KpnI recognition site
 +
|-
 +
|
  
 +
| BBa_K1943013
 +
| Puro CDS
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| 597bp
 +
| Puromycin resistance gene
 +
|-
 +
|
  
 +
| BBa_K1943014
 +
| Bleo CDS
 +
| 369bp
 +
| Resistance gene to antibiotic phleomycin D1
 +
|-
 +
|
  
 +
| BBa_K1943015
 +
| Bla CDS
 +
| 402bp
 +
| Coding sequence of Blasticidin S deaminase, resistant to blasticidin S HCl nucleoside antibiotic
 +
|-
 +
|
 +
 +
| BBa_K1943016
 +
| Bla+E2A
 +
| 456bp
 +
| Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
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|-
 +
|
 +
 +
| BBa_K1943017
 +
| Puro+T2A
 +
| 651bp
 +
| Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
 +
|-
 +
|
 +
 +
| BBa_K1943018
 +
| T2A+Bleo
 +
| 429bp
 +
| Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
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|-
 +
|
 +
 +
| BBa_K1943019
 +
| SV40 promoter + puro anti-antibiotics gene+ 2A + TetOn 3G promoter
 +
| 1774bp
 +
| Tetracycline-responsive promoter, expression induced by doxycycline
 +
|-
 +
|
 +
 +
| BBa_K1943020
 +
| mR-GECO CDS
 +
| 1257bp
 +
| Mutated R-GECO CDS without 2 PstI in original sequence
 +
|-
 +
|
 +
 +
| BBa_K1943021
 +
|
 +
 +
|
 +
 +
|
 +
 +
|-
 +
|
 +
 +
| BBa_K1943022
 +
|
 +
 +
|
 +
 +
|
 +
 +
|-
 +
|
 +
 +
| BBa_K1943023
 +
|
 +
 +
|
 +
 +
|
 +
 +
|-
 +
|
 +
 +
| BBa_K1943024
 +
|
 +
 +
|
 +
 +
|
 +
 +
|-
 +
|
 +
 +
| BBa_K1943025
 +
|
 +
 +
|
 +
 +
|
 +
 +
|-
 +
|
 +
 +
| BBa_K1943026
 +
|
 +
 +
|
 +
 +
|
 +
 +
|-
 +
| Composite Parts
 +
| BBa_K1943000
 +
| J23100+ B0034+ fwYellow+ B0010+ B0012
 +
| 912bp
 +
| Yellow chromoprotein reporter system (Strong Promoter, Strong RBS)
 +
|-
 +
|
 +
 +
| BBa_K1943001
 +
| J23110+ B0031+ fwYellow+ B0010+ B0012
 +
| 914bp
 +
| Yellow chromoprotein reporter system (medium Promoter , weak RBS)
 +
|-
 +
|
 +
 +
| BBa_K1943002
 +
| J23100+ B0034+ gfasPurple + B0010+ B0012
 +
| 867bp
 +
| Purple chromoprotein reporter system (Strong Promoter, Strong RBS)
 +
|-
 +
|
 +
 +
| BBa_K1943003
 +
| J23110+ B0031+ gfasPurple + B0010+ B0012
 +
| 869bp
 +
| Purple chromoprotein reporter system (medium Promoter , weak RBS)
 +
|-
 +
|
 +
 +
| BBa_K1943004
 +
| J23100+ B0034+ eforRed + B0010+ B0012
 +
| 879bp
 +
| Red chromoprotein reporter system (Strong Promoter, Strong RBS)
 +
|-
 +
|
 +
 +
| BBa_K1943005
 +
| J23110+ B0031+ eforRed + B0010+ B0012
 +
| 881bp
 +
| Red chromoprotein reporter system (medium Promoter , weak RBS)
 +
|-
 +
|
 +
 +
| BBa_K1943006
 +
| J23100+ B0034+ spisPink + B0010+ B0012
 +
| 876bp
 +
| Pink chromoprotein reporter system (Strong Promoter, Strong RBS)
 +
|-
 +
|
 +
 +
| BBa_K1943007
 +
| J23110+ B0031+ spisPink + B0010+ B0012
 +
| 878bp
 +
| Pink chromoprotein reporter system (medium Promoter , weak RBS)
 +
|-
 +
|
 +
 +
| BBa_K1943008
 +
| J23116+B0034+sfGFP
 +
| 778bp
 +
| Strong green fluorescence
 +
|-
 +
|
 +
 +
| BBa_K1943009
 +
| J23116+B0034+sfGFP+ B0010+B0012
 +
| 918bp
 +
| Strong green fluorescence
 +
|-
 +
|
 +
 +
| BBa_K19430010
 +
| J23116+B0034+msfGFP
 +
| 781bp
 +
| Strong green fluorescence without KpnI recognition site in CDS
 +
|-
 +
|
 +
 +
| BBa_K19430011
 +
| J23116+B0034+msfGFP+ B0010+B0012
 +
| 915bp
 +
| Strong green fluorescence without KpnI recognition site in CDS
 +
|}
 
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Revision as of 10:13, 18 October 2016

Team SUSTC-Shenzhen

Parts

Contribution


This year, we totally submitted 27 BioBricks, including 15 basic Parts and 12 composite Parts. We always want to contribute all of the components used in our programs and we welcome all researchers to use, measure and modify our Parts for their projects.

We spared no effort do diversify our BioBricks and we successfully constructed BioBricks as listed below:

Type Name Description Length Characteristics
Basic Parts BBa_K1943012 msfGFP CDS 717bp Mutated sfGFP coding sequence without KpnI recognition site
BBa_K1943013 Puro CDS 597bp Puromycin resistance gene
BBa_K1943014 Bleo CDS 369bp Resistance gene to antibiotic phleomycin D1
BBa_K1943015 Bla CDS 402bp Coding sequence of Blasticidin S deaminase, resistant to blasticidin S HCl nucleoside antibiotic
BBa_K1943016 Bla+E2A 456bp Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
BBa_K1943017 Puro+T2A 651bp Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
BBa_K1943018 T2A+Bleo 429bp Anti-antibiotics genes CDS with 2A sequence for selection when ligated with core sequence
BBa_K1943019 SV40 promoter + puro anti-antibiotics gene+ 2A + TetOn 3G promoter 1774bp Tetracycline-responsive promoter, expression induced by doxycycline
BBa_K1943020 mR-GECO CDS 1257bp Mutated R-GECO CDS without 2 PstI in original sequence
BBa_K1943021
BBa_K1943022
BBa_K1943023
BBa_K1943024
BBa_K1943025
BBa_K1943026
Composite Parts BBa_K1943000 J23100+ B0034+ fwYellow+ B0010+ B0012 912bp Yellow chromoprotein reporter system (Strong Promoter, Strong RBS)
BBa_K1943001 J23110+ B0031+ fwYellow+ B0010+ B0012 914bp Yellow chromoprotein reporter system (medium Promoter , weak RBS)
BBa_K1943002 J23100+ B0034+ gfasPurple + B0010+ B0012 867bp Purple chromoprotein reporter system (Strong Promoter, Strong RBS)
BBa_K1943003 J23110+ B0031+ gfasPurple + B0010+ B0012 869bp Purple chromoprotein reporter system (medium Promoter , weak RBS)
BBa_K1943004 J23100+ B0034+ eforRed + B0010+ B0012 879bp Red chromoprotein reporter system (Strong Promoter, Strong RBS)
BBa_K1943005 J23110+ B0031+ eforRed + B0010+ B0012 881bp Red chromoprotein reporter system (medium Promoter , weak RBS)
BBa_K1943006 J23100+ B0034+ spisPink + B0010+ B0012 876bp Pink chromoprotein reporter system (Strong Promoter, Strong RBS)
BBa_K1943007 J23110+ B0031+ spisPink + B0010+ B0012 878bp Pink chromoprotein reporter system (medium Promoter , weak RBS)
BBa_K1943008 J23116+B0034+sfGFP 778bp Strong green fluorescence
BBa_K1943009 J23116+B0034+sfGFP+ B0010+B0012 918bp Strong green fluorescence
BBa_K19430010 J23116+B0034+msfGFP 781bp Strong green fluorescence without KpnI recognition site in CDS
BBa_K19430011 J23116+B0034+msfGFP+ B0010+B0012 915bp Strong green fluorescence without KpnI recognition site in CDS

Made by from the iGEM team SUSTech_Shenzhen.

Licensed under CC BY 4.0.

  • ©2016 SUSTech_Shenzhen