Difference between revisions of "Team:British Columbia/Composite Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Composite Part special prize</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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        <strong><p style="font-size: 3em">Composite Part</p></strong>
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<strong>
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<a href="https://2016.igem.org/Team:British_Columbia">Home</a> /
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<a href="https://2016.igem.org/Team:British_Columbia/Composite_Part">Achievements - Parts - Composite Part</a></strong>
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<h1>Favorite Composite Part: BBa_k2139005</h1>
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<img class="img-center img-responsive" style="display: table; margin: 0 auto" src="https://static.igem.org/mediawiki/2016/a/a0/T--British_columbia--Composite.png">
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<p align="justify"><a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2139005"> BBa_K2139005</a> is the expression cassette of the basic part <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2139003"> BBa_K2139003</a> that was used to do the surface expression experiments in <i>C. crescentus</i>. Although we were un-able to functionally characterize it during our project due to time constraints (characterized as fusion protein see data here), it has been well documented in literature which can be found below.</p>
  
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<p align="justify">The construct was designed to contain the Ptac promotor, ribosome binding site, coding region, and double terminator. This was to allow for the constitutive expression of the enzyme. The catalytic function of the enzyme is to convert cellulose to cellobiose (below).
  
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<p align="justify"><b>References:</b> Linger, Jeffrey G., William S. Adney, and Al Darzins. "Heterologous Expression and Extracellular Secretion of Cellulolytic Enzymes by Zymomonas Mobilis." Applied and Environmental Microbiology, vol. 76, no. 19, 2010., pp. 6360-6369doi:10.1128/AEM.00230-10.
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<h4>Note</h4>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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<p id="read-more"><strong>Check out other parts of our project below!</strong></p>
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<a href="https://2016.igem.org/Team:British_Columbia/Achievements/Parts">
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<img src="https://static.igem.org/mediawiki/2016/6/60/T--British_Columbia--header-mountains.jpg"></a>
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<a href="https://2016.igem.org/Team:British_Columbia/Project/S-Layer/Cellulases">
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<strong><figcaption>Part List</figcaption></strong></a>
 
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<a href="https://2016.igem.org/Team:British_Columbia/Project/S-Layer/Cellulases">
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<strong><figcaption>Cellulases</figcaption></strong></a></div>
 
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Revision as of 14:50, 18 October 2016

Main CSS Navbar CSS

Composite Parts

Composite Part

Favorite Composite Part: BBa_k2139005

BBa_K2139005 is the expression cassette of the basic part BBa_K2139003 that was used to do the surface expression experiments in C. crescentus. Although we were un-able to functionally characterize it during our project due to time constraints (characterized as fusion protein see data here), it has been well documented in literature which can be found below.

The construct was designed to contain the Ptac promotor, ribosome binding site, coding region, and double terminator. This was to allow for the constitutive expression of the enzyme. The catalytic function of the enzyme is to convert cellulose to cellobiose (below).

References: Linger, Jeffrey G., William S. Adney, and Al Darzins. "Heterologous Expression and Extracellular Secretion of Cellulolytic Enzymes by Zymomonas Mobilis." Applied and Environmental Microbiology, vol. 76, no. 19, 2010., pp. 6360-6369doi:10.1128/AEM.00230-10.

Check out other parts of our project below!