Difference between revisions of "Team:ShanghaitechChina/Description"
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<ul id="sidebar" class="nav bs-docs-sidenav "> | <ul id="sidebar" class="nav bs-docs-sidenav "> | ||
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| − | <a href="#p1">Improve the characterization</a> | + | <a href="#p1"><h5>Improve the characterization</h5></a> |
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| − | <a href="#p2">Optimize the codon</a> | + | <a href="#p2"><h5>Optimize the codon</h5></a> |
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<h4>Details see ShanghaitechChina team's <a href="https://2016.igem.org/Team:ShanghaitechChina/Notebook#biofilm">protocol</a></h4> | <h4>Details see ShanghaitechChina team's <a href="https://2016.igem.org/Team:ShanghaitechChina/Notebook#biofilm">protocol</a></h4> | ||
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</div> | </div> | ||
</div> | </div> | ||
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<h1 align="center">Optimize the codon</h1> | <h1 align="center">Optimize the codon</h1> | ||
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<div class="col-lg-12"> | <div class="col-lg-12"> | ||
<h4>2.Parts Collection Two (Hydrogenase gene clusters)</h4> | <h4>2.Parts Collection Two (Hydrogenase gene clusters)</h4> | ||
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<h6>The original sequences of hydrogenase were found in <a href="www.genome.jp">www.genome.jp</a>. With the help of OptimumGene™, We used the following parameters to optimize our gene sequences without changing their amino acids sequence: Codon usage bias, GC content, CpG dinucleotides content, mRNA secondary structure, Cryptic splicing sites, Premature PolyA sites, Internal chi sites and ribosomal binding sites, Negative CpG islands, RNA instability motif (ARE), Repeat sequences (direct repeat, reverse repeat, and Dyad repeat).</h6> | <h6>The original sequences of hydrogenase were found in <a href="www.genome.jp">www.genome.jp</a>. With the help of OptimumGene™, We used the following parameters to optimize our gene sequences without changing their amino acids sequence: Codon usage bias, GC content, CpG dinucleotides content, mRNA secondary structure, Cryptic splicing sites, Premature PolyA sites, Internal chi sites and ribosomal binding sites, Negative CpG islands, RNA instability motif (ARE), Repeat sequences (direct repeat, reverse repeat, and Dyad repeat).</h6> | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Revision as of 02:54, 19 October 2016
