Difference between revisions of "Team:ShanghaitechChina/Demonstrate"
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| − | <a href="#Demonstration ">Demonstration | + | <a href="#Demonstration ">Demonstration</a> |
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It contains (1) an anaerobic reaction container which is a transparent circular cuvette that allows light to go through; (2) a light source in our hydrogen production assay acting as a substitute for the real sun. (We chose a high-power white LED light, set 28cm away from the reaction container for a even distribution of photons); (3) a hydrogen electrode linked to its inner sensor inserted into the reaction container to measure the realtime concentration of hydrogen; (4) a date hub; (5) a computer connected to the hub to record the data and generate the curve of concentration variation within a period of time. <p></p> | It contains (1) an anaerobic reaction container which is a transparent circular cuvette that allows light to go through; (2) a light source in our hydrogen production assay acting as a substitute for the real sun. (We chose a high-power white LED light, set 28cm away from the reaction container for a even distribution of photons); (3) a hydrogen electrode linked to its inner sensor inserted into the reaction container to measure the realtime concentration of hydrogen; (4) a date hub; (5) a computer connected to the hub to record the data and generate the curve of concentration variation within a period of time. <p></p> | ||
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| + | <center> <h1 id="AResults"> Results</h1></center> | ||
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<center><img src="https://static.igem.org/mediawiki/2016/4/4f/T--ShanghaitechChina--asasy-withfinalplan-bidirectlycat.png"></center> | <center><img src="https://static.igem.org/mediawiki/2016/4/4f/T--ShanghaitechChina--asasy-withfinalplan-bidirectlycat.png"></center> | ||
| − | <p style="text-align:center"><b>Figure | + | <p style="text-align:center"><b>Figure 2</b> Hydrogen production with nano rods suspension replaced by nano rods bound to biofilm beads.</p> |
During the period with lighting, the hydrogen production increases, until we shut off the light at points that correspond to the tips. The curve then goes downward, showing that the hydrogen concentration is lowered, an evidence of the bidirectional catalytic activity of hydrogenase. It is noteworthy that the hydrogenase shows the greatest production rate at the beginning of lighting: a transient sharp rise can be observed at the valleys. It is also obvious that each period of “light-on light-off” gives similar curves, which implies that our hydrogenase is stable. | During the period with lighting, the hydrogen production increases, until we shut off the light at points that correspond to the tips. The curve then goes downward, showing that the hydrogen concentration is lowered, an evidence of the bidirectional catalytic activity of hydrogenase. It is noteworthy that the hydrogenase shows the greatest production rate at the beginning of lighting: a transient sharp rise can be observed at the valleys. It is also obvious that each period of “light-on light-off” gives similar curves, which implies that our hydrogenase is stable. | ||
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| − | <b>Comparing the system with biofilm and with out biofilm Figure | + | <b>Comparing the system with biofilm and with out biofilm Figure 2 and Figure 3</b><p></p> |
| − | Before we tested the system will biofilm-anchored CdS nanorods, we tested ones with freely-flowing CdS nanorods. The result is shown below in | + | Before we tested the system will biofilm-anchored CdS nanorods, we tested ones with freely-flowing CdS nanorods. The result is shown below in Figure3. <p></p> |
During lighting period, the hydrogen production increases, until we shut off the light at points that correspond to the tips. The curve then goes downward, showing that the hydrogen concentration is lowered, an evidence of bidirectional catalytic activity of hydrogenase. <center><img src="https://static.igem.org/mediawiki/2016/a/ab/T--ShanghaitechChina--asasy--bidirectlycat.png"></center> | During lighting period, the hydrogen production increases, until we shut off the light at points that correspond to the tips. The curve then goes downward, showing that the hydrogen concentration is lowered, an evidence of bidirectional catalytic activity of hydrogenase. <center><img src="https://static.igem.org/mediawiki/2016/a/ab/T--ShanghaitechChina--asasy--bidirectlycat.png"></center> | ||
| − | In the process of hydrogen generation without biofilm-anchored CdS, a stir bar with a necessary speed of 800 RPM was needed. But in Figure | + | In the process of hydrogen generation without biofilm-anchored CdS, a stir bar with a necessary speed of 800 RPM was needed. But in Figure 2, the system with biofilm, a stir bar was not used. It is likely because the aggregates of NR have a bigger chance in colliding with <em>E. coli</em> to transfer electrons. We therefore propose this model as our final model, although further optimization of the system is still under way, including deciding on the optimized material and size the microsphere for biofilm growth. <p></p> |
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Revision as of 10:45, 19 October 2016
