Difference between revisions of "Team:British Columbia/Composite Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Composite Part special prize</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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      <div class="col-sm-12">
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        <strong><p style="font-size: 3em">Composite Part</p></strong>
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<strong>
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<a href="https://2016.igem.org/Team:British_Columbia">Home</a> /
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<a href="https://2016.igem.org/Team:British_Columbia/Composite_Part">Achievements - Composite Part</a></strong>
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<div class="content-wrap">
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<h1>Favorite Composite Part: BBa_k2139005</h1>
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<img class="img-center img-responsive" style="display: table; margin: 0 auto" src="https://static.igem.org/mediawiki/2016/e/e8/T--British_columbia--gluc1c.png">
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<p align="justify"><a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2139005"> BBa_K2139005</a> is the expression cassette of the basic part <a href= "http://parts.igem.org/wiki/index.php?title=Part:BBa_K2139002"> BBa_K2139002</a> that was used to do the surface expression experiments in <i>C. crescentus</i>. We were able to functionally characterize it during our project and you can see the data <a href= "https://2016.igem.org/Team:British_Columbia/Project/S-Layer/Cellulases"> here</a> , it has been well documented in literature which can be found below. It originates from the microorganism <i>Paenibacillus sp. MTCC 5639 </i> and the part has been codon optimized for expression in <i>C. crescentus</i>. As such, it contains a high GC content and shows significant difficulty in being amplified by PCR.
  
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<p align="justify">The construct was designed to contain the Ptac promotor, ribosome binding site, coding region, and double terminator. This was to allow for the constitutive expression of the enzyme. The catalytic function of the enzyme is to convert cellulose to cellobiose (below).
  
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<p align="justify"><b>References:</b> Gupta, S., Adlakha, N., & Yazdani, S. S. (2013). Efficient extracellular secretion of an endoglucanase and a β-glucosidase in E. coli. Protein Expression and Purification, 88(1), 20-25. doi:10.1016/j.pep.2012.11.006.
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<p id="read-more"><strong>Check out other parts of our project below!</strong></p>
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<a href="https://2016.igem.org/Team:British_Columbia/Project/S-Layer/Laccases">
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<img src="https://static.igem.org/mediawiki/2016/1/1a/T--British_Columbia--header-laccases.JPG"></a>
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<a href="https://2016.igem.org/Team:British_Columbia/Project/S-Layer/Laccases">
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<strong><figcaption>Laccases</figcaption></strong></a>
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<h4>Note</h4>
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<a href="https://2016.igem.org/Team:British_Columbia/Project/Bio-Pathways">
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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<img src="https://static.igem.org/mediawiki/2016/7/76/T--British_Columbia--header-branches.JPG"></a>
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<a href="https://2016.igem.org/Team:British_Columbia/Project/Bio-Pathways">
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<strong><figcaption>Biosynthetic Pathways</figcaption></strong></a>
 
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Latest revision as of 11:50, 19 October 2016

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Composite Parts

Composite Part

Favorite Composite Part: BBa_k2139005

BBa_K2139005 is the expression cassette of the basic part BBa_K2139002 that was used to do the surface expression experiments in C. crescentus. We were able to functionally characterize it during our project and you can see the data here , it has been well documented in literature which can be found below. It originates from the microorganism Paenibacillus sp. MTCC 5639 and the part has been codon optimized for expression in C. crescentus. As such, it contains a high GC content and shows significant difficulty in being amplified by PCR.

The construct was designed to contain the Ptac promotor, ribosome binding site, coding region, and double terminator. This was to allow for the constitutive expression of the enzyme. The catalytic function of the enzyme is to convert cellulose to cellobiose (below).

References: Gupta, S., Adlakha, N., & Yazdani, S. S. (2013). Efficient extracellular secretion of an endoglucanase and a β-glucosidase in E. coli. Protein Expression and Purification, 88(1), 20-25. doi:10.1016/j.pep.2012.11.006.

Check out other parts of our project below!