Difference between revisions of "Team:SUSTech Shenzhen/Model/Calibration"

 
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      url=wiki/images/6/61/T--SUSTech_Shenzhen--LOGO_nowords.png|size=100px|
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      title=Calibration|
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      subtitle=Calibration of Flow Speed}}
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= Calibration of fluid flow velocity =
 
= Calibration of fluid flow velocity =
  
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To visualize the flow profile, rainbow beads (diameter:) were dissolved in the cell culture medium, which contains the dead cells. A series of pump speed(5ul/min, 15ul/min, and 45ul/min) were applied to generate a steady fluid flow. The exposure time was set to 100m so that the length of the streak(shown as a gray line in Fig. ) represents the total traveling distance of the beads during the exposure time. Fig. shows a typical image of a bead trace in the fast microfluidics channel with a pump speed of 5ul/min. To minimize hydraulic effects of the PDMS walls, the pictures were taken from the midplane of the channel providing the maximum flow velocity. Note that the traveling direction of the beads was left. Each streak was measured separately, and the average length of all streaks in one particular channel divides the exposure time was regarded as the fluid flow velocity of this channel. <ref>Maneshi MM, Sachs F, Hua SZ, ''A Threshold Shear Force for Calcium Influx in an Astrocyte Model of Traumatic Brain Injury.'' J Neurotrauma. 2015 Jul 1, '''32'''(13) : p. 1020-9. </ref>
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To visualize the flow profile, rainbow beads (SPHERO<sup>TM</sup> Rainbow Calibration Particles, Cat No. RCP-60-5, Lot No. AB01, diameter: 6um<ref>{{SUSTech_Shenzhen/cite web | url=http://www.spherotech.com | title= Rainbow beads official website}}</ref>) were dissolved in the cell culture medium, which contained some dead cells. A series of pumped flow rate(5μl/min, 15μl/min, and 45μl/min) were applied to generate a steady fluid flow. The length of the streakline (shown as a gray line in picture) represents the total traveling distance of the beads during the exposure time of 100ms . Fig. 1 shows a typical image of a bead trace(fastest microfluidics channel with the flow rate of 5ul/min). To minimize hydraulic effects of the PDMS walls, the pictures were taken from the midplane of the channel providing the maximum flow velocity. Note that the traveling direction of the beads was left. Each streak was measured separately, and the average length of all streaks in one particular channel divides the exposure time was regarded as the maximum flow velocity of this channel. <ref>Maneshi MM, Sachs F, Hua SZ, ''A Threshold Shear Force for Calcium Influx in an Astrocyte Model of Traumatic Brain Injury.''J Neurotrauma. 2015 Jul 1, '''32'''(13) : p. 1020-9. </ref>
  
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Fig. [num]: a typical method to measure the fluid flow velocity.
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{{SUSTech_Image | filename=T--SUSTech_Shenzhen--DB56D45F-D056-4A82-897A-2CE77C2C4A12.png | caption='''Fig.1 Bead Trace in The Fastest Microfluidics Channel with The Flow Rate of 5ul/min''' | width=1000px}}
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== Experiment ==
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To minimize the error, we took pictures on fast microfluidics channel when pumped flow rate was 5μl/min; on mid microfluidics channel when pumped flow rate was 15μl/min; on slow microfluidics channel when pumped flow rate was 45μl/min, considering that fluid flow velocity is proportional to pumped flow rate.
  
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The data of streak length is represented in the table below:
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Data collection blow:
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{| class="table table-striped"
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{| class=“table table-striped”
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! channel !! flow rate(μl/min) !! length①(um) !! length②(um) !! length③(um)  
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! channel !! speed(ul/min) !! length①(um) !! length②(um) !! length③(um)  
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|-
 
|-
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! fast || 5 || 630 || 455 || 610
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| fast || 5 || 630 || 455 || 610
 
|-
 
|-
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! mid || 15 || 180 || 160 || 180
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| mid || 15 || 180 || 160 || 180
 
|-
 
|-
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| slow || 54 || 48 || 75 || 80  
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| slow || 45 || 48 || 75 || 80  
 
|}
 
|}
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== Result ==
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We wrote a MatLab program to calculate the results(fluid flow velocity). Codes were shown below:
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We wrote a MatLab program to calculate the maximum flow velocities. Codes were shown below:
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<html><div class="bs-callout bs-callout-info">
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<code>
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<h4>MatLab Code:</h4>
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ex_fast=[630,455,610]*90;  % align the data to um/s by multiply 10 and to  
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<p>
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ex_fast=[630,455,610]*90;   
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</p><p>
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% align the data to um/s by multiply 10 and convert to 45μl/min
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</p><p>
 
ex_mid=[180,160,180]*30;
 
ex_mid=[180,160,180]*30;
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</p><p>
 
ex_slow=[48,75,80]*10;
 
ex_slow=[48,75,80]*10;
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</p><p>
 
ex_fast_mean=mean(ex_fast); ex_fast_std=std(ex_fast);
 
ex_fast_mean=mean(ex_fast); ex_fast_std=std(ex_fast);
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</p><p>
 
ex_mid_mean=mean(ex_mid); ex_mid_std=std(ex_mid);
 
ex_mid_mean=mean(ex_mid); ex_mid_std=std(ex_mid);
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</p><p>
 
ex_slow_mean=mean(ex_slow); ex_slow_std=std(ex_slow);
 
ex_slow_mean=mean(ex_slow); ex_slow_std=std(ex_slow);
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</code>
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</p>
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</div>
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</html>
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Note: ex_fast_mean is the mean value of maximum flow velocity of fast microfluidics channel when pumped flow rate is 45μl/min, ex_fast_std is its standard deviation, et cetera.
  
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Note: ex_fast_mean is the mean value of fluid flow velocity of fast microfluidics channel when pump speed is 45ul/min, ex_fast_std is its standard deviation, et cetera.
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{| class="table table-striped"
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! variable !! mean(um/s) !! variable !! std(um/s)
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The results were shown below:
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{| class=“table table-striped”
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! variable !! mean(ul/min) !! variable !! std(ul/min)
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|-
 
|-
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! ex_fast_mean || 50850 !! ex_fast_std || 8620.8
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| ex_fast_mean || 50850 || ex_fast_std || 8600
 
|-
 
|-
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! ex_mid_mean || 5200 !! ex_mid_std || 346.4102
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| ex_mid_mean || 5200 || ex_mid_std || 350
 
|-
 
|-
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| ex_slow_mean || 676.67 !! ex_slow_std || 172.14
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| ex_slow_mean || 676 || ex_slow_std || 170
 
|}
 
|}
  
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[[柱状errorbar图 3通道 泵速45ul/min]]
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The ratio of maximum velocities in 3 different channels is 13:100:978.
  
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Fig. [num] the graph of fluid flow velocity results under the pump speed of 45ul/min. It shows that the velocity rate of 3 channels is 13:100:978.
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{{SUSTech_Image | filename=T--SUSTech_Shenzhen--855C7E02-2E51-441A-9492-A2B281185010.png | caption='''Fig.2 Maximum Velocities in Each Channel with The Flow Rate of 45ul/min''' | width=1000px}}
  
 
= Reference =
 
= Reference =
  
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<reference/>
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<references/>
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Latest revision as of 12:10, 19 October 2016

Team SUSTC-Shenzhen

Calibration

Calibration of Flow Speed

Calibration of fluid flow velocity

To visualize the flow profile, rainbow beads (SPHEROTM Rainbow Calibration Particles, Cat No. RCP-60-5, Lot No. AB01, diameter: 6um[1]) were dissolved in the cell culture medium, which contained some dead cells. A series of pumped flow rate(5μl/min, 15μl/min, and 45μl/min) were applied to generate a steady fluid flow. The length of the streakline (shown as a gray line in picture) represents the total traveling distance of the beads during the exposure time of 100ms . Fig. 1 shows a typical image of a bead trace(fastest microfluidics channel with the flow rate of 5ul/min). To minimize hydraulic effects of the PDMS walls, the pictures were taken from the midplane of the channel providing the maximum flow velocity. Note that the traveling direction of the beads was left. Each streak was measured separately, and the average length of all streaks in one particular channel divides the exposure time was regarded as the maximum flow velocity of this channel. [2]

T--SUSTech Shenzhen--DB56D45F-D056-4A82-897A-2CE77C2C4A12.png
Fig.1 Bead Trace in The Fastest Microfluidics Channel with The Flow Rate of 5ul/min

Experiment

To minimize the error, we took pictures on fast microfluidics channel when pumped flow rate was 5μl/min; on mid microfluidics channel when pumped flow rate was 15μl/min; on slow microfluidics channel when pumped flow rate was 45μl/min, considering that fluid flow velocity is proportional to pumped flow rate.

Data collection blow:

channel flow rate(μl/min) length①(um) length②(um) length③(um)
fast 5 630 455 610
mid 15 180 160 180
slow 45 48 75 80

Result

We wrote a MatLab program to calculate the maximum flow velocities. Codes were shown below:

MatLab Code:

ex_fast=[630,455,610]*90;

% align the data to um/s by multiply 10 and convert to 45μl/min

ex_mid=[180,160,180]*30;

ex_slow=[48,75,80]*10;

ex_fast_mean=mean(ex_fast); ex_fast_std=std(ex_fast);

ex_mid_mean=mean(ex_mid); ex_mid_std=std(ex_mid);

ex_slow_mean=mean(ex_slow); ex_slow_std=std(ex_slow);

Note: ex_fast_mean is the mean value of maximum flow velocity of fast microfluidics channel when pumped flow rate is 45μl/min, ex_fast_std is its standard deviation, et cetera.

variable mean(um/s) variable std(um/s)
ex_fast_mean 50850 ex_fast_std 8600
ex_mid_mean 5200 ex_mid_std 350
ex_slow_mean 676 ex_slow_std 170

The ratio of maximum velocities in 3 different channels is 13:100:978.

T--SUSTech Shenzhen--855C7E02-2E51-441A-9492-A2B281185010.png
Fig.2 Maximum Velocities in Each Channel with The Flow Rate of 45ul/min

Reference

  1. ↑ Rainbow beads official website, Retrieved from http://www.spherotech.com.
  2. ↑ Maneshi MM, Sachs F, Hua SZ, A Threshold Shear Force for Calcium Influx in an Astrocyte Model of Traumatic Brain Injury.J Neurotrauma. 2015 Jul 1, 32(13) : p. 1020-9.

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